在微塑料诱导的心脏损伤中,N6-甲基氨酸甲基化介导非编码RNA的修饰
Min Zhang1, Jun Shi2, Jun Zhou1
1Division of Cardiology, Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 200336 Shanghai, China.
Ecotoxicology and environmental safety
|June 24, 2023
概括
微塑料 (MPs) 通过改变非编码RNA (ncRNA) 和它们的N6-甲基氨酸 (m6A) 修饰而导致心脏损伤. 这项研究揭示了治疗MP诱导心脏毒性的新分子标.
科学领域:
- 环境健康 环境健康
- 分子生物学分子生物学
- 心血管研究研究心血管研究
背景情况:
- 全球微塑料 (MP) 污染引发了健康问题,但MP毒性机制尚不清楚.
- 非编码RNAs (ncRNAs) 与MP血管毒性有关,而N6-甲基氨酸 (m6A) 修饰影响心血管疾病.
- MP诱导的心脏毒性对ncRNA m6A修饰的影响仍然未知.
研究的目的:
- 研究MP诱导心脏毒性期间ncRNA中m6A修饰的作用.
- 在暴露于MP的心肌组织中分析差异表达的ncRNA及其m6A修饰模式.
- 为了确定潜在的分子标,以减轻MP诱导的心脏损伤.
主要方法:
- 使用了RNA测序 (RNA-seq) 和甲基化RNA免疫沉测序 (MeRIP-seq).
- 在暴露于MP的器官中评估了MP的积累,亡和m6A/METTL3水平.
- 分析了长非编码RNA (lncRNA) 和圆形RNA (circRNA) 的差异表达和m6A修饰.
主要成果:
- 在器官中积累的MPs增加了细胞亡和心肌m6A/METTL3水平.
- 在MP治疗心脏中,有392个lncRNA和302个circRNA被差异表达.
- 暴露于MP改变了ncRNA中的m6A修饰模式,circ-Arfgef2和lncG3bp2显示上调.
结论:
- 由MP引起的心脏毒性涉及ncRNA表达和m6A修饰的改变.
- 上调的m6A修饰和特定的ncRNA如circ-Arfgef2和lncG3bp2与MP心脏损伤有关.
- 结果提供了对MP心脏毒性机制的见解,并确定了潜在的治疗点.
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