微RNAmiR-152可以支持卵巢颗粒细胞功能,并修改apigenin的作用
Z Fabová1, Z Kislíková1, B Loncová1
1Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovakia.
Domestic animal endocrinology
|June 24, 2023
概括
原蛋白通过增加microRNA-152 (miR-152) 的表达,增强卵巢颗粒细胞功能. 这项研究显示了miR-152的存在.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 细胞生理学 细胞生理学
背景情况:
- 卵巢颗粒细胞对于生殖功能至关重要.
- 越来越多地认识到特定的microRNAs,如miR-152,在颗粒细胞活动中的作用.
- 如阿皮基宁等黄类药物正在研究它们对细胞过程的潜在调节作用.
研究的目的:
- 调查原蛋白和microRNA-152 (miR-152) 在调节猪粒状细胞功能中的作用.
- 阐明阿皮基宁与miR-152在控制卵巢细胞行为的相互关系.
- 分析原蛋白和miR-152调制对细胞活力,增殖,亡和荷尔蒙释放的影响.
主要方法:
- 猪粒状细胞被用不同度的apigenin处理.
- 研究了miR-152类似物和抑制剂单独或与阿皮金因结合的作用.
- 分析包括基因表达 (miR-152),细胞活力试验,增殖试验,亡试验以及类固醇激素 (雌激素,孕),IGF-I,催产素和前列腺素E2释放量的量化.
主要成果:
- 原蛋白治疗显著增加了miR-152表达,细胞增殖和雌激醇释放,同时降低了细胞亡,孕激素和IGF-I输出.
- miR-152类似物促进了细胞活力,增殖和孕激素,IGF-I,催产素和前列腺素E2的释放,但抑制了细胞亡和雌激素.
- miR-152 抑制剂表现出相反的效果. miR-152类似物也调节了阿比基宁对亡和雌醇释放的影响.
结论:
- 原蛋白在调节基本的卵巢颗粒细胞功能方面发挥着重要作用.
- 这项研究提供了第一个证据,证明miR-152对于控制这些卵巢功能至关重要.
- 原蛋白促进miR-152的表达,miR-152反过来影响原蛋白对卵巢细胞的影响,突出了新的调节途径.
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