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人类的过氧体NAD+/NADH稳态受两个独立的NAD (H) 穿系统的调节

Serhii Chornyi¹, Cláudio F Costa², Lodewijk IJlst³

¹Amsterdam UMC - University of Amsterdam, Department of Clinical Chemistry, Laboratory Genetic Metabolic Diseases, Meibergdreef 9, 1105 AZ, Amsterdam, the Netherlands; Amsterdam Gastroenterology Endocrinology Metabolism, Amsterdam, the Netherlands.

Free radical biology & medicine

|June 24, 2023

概括

人类过氧体通过两种新的穿系统保持其氧化还原平衡,其中包括乳酸和酸脱酶. 细胞醇和过氧体之间的这种交叉对细胞生物能学和NAD (H) 恒温至关重要.

科学领域:

细胞生物学细胞生物学
代谢生物化学代谢生物化学
遗传学是一个遗传学.

背景情况:

尼古丁胺胺氨基二核酸 (NAD(H)) 是细胞生物能学的必要辅助因子.
过氧体是参与脂质代谢的重要器官,含有依赖NAD (H) 的酶.
过氧体内NAD (H) 稳态的调节至关重要,但人们对其了解甚少.

研究的目的:

为了研究人体氧体中NAD (H) 恒温的调节.
开发用于测量内氧体内NADH水平的方法.
为了确定连接细胞和过氧体NAD (H) 比率的机制.

主要方法:

开发用于人类细胞的氧体向的NADH生物传感器.
用CRISPR-Cas9基因组编辑来研究基因功能.
在细胞质和过氧体中测量NAD+/NADH比率.

主要成果:

已确立的特定测量内氧体内NADH的方法.
证明了细胞和过氧体NAD+/NADH比率之间的密切联系.
确定了通过停止编码子读透生成的内乳酸盐和酸盐脱酶,作为这种交叉语音的媒介.
提供了两个独立的氧化还原穿系统在人体过氧体中的证据.

结论:

人类的过氧体具有两种不同的氧化还原穿系统,调节NAD (H) 稳态.
通过翻译性停止密码子读取生成的蛋白质异型在人类中具有特定的代谢功能.
这项研究阐明了一种维持过氧体代谢功能和细胞生物能学的新机制.

关键词:

在β-氧化过程中.生物能源学生物能源学辅助因子辅助因子辅助因子脱水酶可以脱水.代谢过程中的代谢.复氧化平衡还原平衡.

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