LncDGAT2是山羊脂肪细胞热生成基因程序的新型正调节剂
Xin Liu1, Chunhua Huang1, Tingting Jiang1
1Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China.
International journal of biological macromolecules
|June 24, 2023
概括
暴露于寒冷会诱导新生山羊的长非编码RNAs (lncRNAs),其中lncDGAT2促进脂肪细胞分化和发热. 在这个过程中,PRDM4-lncDGAT2通路至关重要.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 代谢调节 代谢调节 代谢调节 代谢调节
背景情况:
- 棕色和色脂肪组织热生成对于新生哺乳动物的温度调节至关重要.
- 新生儿中调节热生成脂肪的分子机制,特别是长非编码RNA (lncRNAs),在很大程度上是未知的.
- 暴露于寒冷是影响脂肪组织功能的关键环境因素.
研究的目的:
- 为了研究 lncRNAs 在调节新生山羊在寒冷暴露后脂肪热生成中的作用.
- 为了确定参与棕色脂肪组织 (BAT) 寒冷诱导的热生成的特定lncRNAs.
- 阐明新生儿发热过程中涉及lncRNAs和转录因子的调节途径.
主要方法:
- 在新生的山羊中识别冷诱导的lncRNA.
- 在暴露于寒冷后,对lncRNA丰富的定量分析.
- 关于脂肪细胞分化和发热基因表达的功能研究.
- 对转录因子与lncRNA促进体结合的研究.
主要成果:
- 鉴定出 lncDGAT2 是一种 lncRNA,在暴露在寒冷环境后,其在 BAT 中得到显著丰富.
- 发现 lncDGAT2 能够促进棕色和白色脂肪细胞的分化.
- lncDGAT2增强了发热基因的表达.
- 转录因子PRDM4直接激活了lncDGAT2转录.
- 证明PRDM4-lncDGAT2轴对棕色脂肪细胞热生成基因程序至关重要.
结论:
- lncRNAs,特别是IncDGAT2,在调节新生哺乳动物脂肪热生成方面发挥着重要作用.
- 在棕色脂肪细胞中,PRDM4-lncDGAT2调节轴对于激活热生成基因程序至关重要.
- 这些发现突出了新鲜的lncRNA和转录因子相互作用,用于控制新生儿能量代谢和温度调节.
相关概念视频
01:09GPCRs Regulate Adenylyl Cylase Activity
5.7K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.7K
02:23Master Transcription Regulators
7.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.0K
01:58Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
01:16TGF - β Signaling Pathway
7.5K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.5K
02:39lncRNA - Long Non-coding RNAs
8.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K