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Updated: Jul 25, 2025

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
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综合系统 (2D PLE×HPLC-PDA) 的应用,用于生物活性化合物提取和在线量化:优点,验证和考虑

Juliane Viganó1, Monique Martins Strieder2, Rodrigo S Pizani2

  • 1Centro de Ciências da Natureza, Universidade Federal de São Carlos, Rod. Lauri Simões de Barros, Km 12 - SP 189, Buri, SP, 18290-000, Brazil; Multidisciplinary Laboratory of Food and Health (LabMAS), School of Applied Sciences (FCA), Universidade Estadual de Campinas, São Paulo, Brazil.

Analytica chimica acta
|June 24, 2023
PubMed
概括

一个新的2D加压液体提取 (PLE) ×高性能液体染色学与光二极管阵列检测 (HPLC-PDA) 系统集成了固体样品制备和生物活性化合物分析. 这种经过验证的方法可以在线量化食品副产品中的化合物,提供一种可持续的分析方法.

关键词:
替代技术是一种替代技术.生物化合物 生物化合物在线分析分析在线分析量化提取 量化提取 量化提取实时提取评估实时提取评估样品的准备 样品的准备

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High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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