使用Iqgap3-2A-CreERT2小鼠识别成年胃组织干细胞/祖细胞
Junichi Matsuo1, Linda Shyue Huey Chuang1, Jasmine Jie Lin Tong1
1Cancer Science Institute of Singapore, National University of Singapore, Singapore, Singapore.
Methods in molecular biology (Clifton, N.J.)
|June 24, 2023
概括
研究人员将Iqgap3确定为小鼠胃中的恒常干细胞标记物. 针对这些Iqgap3表达细胞的瘤性KrasG12D迅速诱导癌前转化,这表明它们在早期癌症发生中的作用.
科学领域:
- 胃肠病学 胃肠病学
- 干细胞生物学 干细胞生物学
- 在瘤学瘤学.
背景情况:
- 识别正常和癌症干细胞的独特基因标记物有助于理解瘤的起源和行为.
- 在小鼠模型中的血统追踪对于识别成年干细胞及其活体活动至关重要.
- 最近,Iqgap3被确定为小鼠胃中恒常干细胞标记物.
研究的目的:
- 详细介绍一个谱系追踪协议,用于评估小鼠胃中的干细胞活性.
- 研究Iqgap3表达的恒常干细胞在致癌发生过程中作为起源细胞的作用.
- 为了追踪早期细胞变化之前的瘤转化.
主要方法:
- 使用Cre重组酶驱动的CreERT2记者小鼠模型进行血统追踪.
- 使用Iqgap3-2A-CreERT2;Rosa26-LSL-td番茄小鼠模型跟踪Iqgap3表达细胞.
- 在Iqgap3表达细胞中诱导瘤性KrasG12D表达,以研究致癌性.
主要成果:
- 血统追踪证实了Iqgap3表达细胞中的干细胞活性.
- 在Iqgap3表达细胞中KrasG12D的诱导导致了癌前代谢的快速发展.
- 异常的Iqgap3表达细胞被认为是早期胃癌发生的关键决定因素.
结论:
- 伊卡普3作为可靠的标志物用于小鼠胃中的恒温干细胞.
- 表达Iqgap3的细胞被认为是胃癌的潜在来源细胞.
- 这项研究为研究干细胞在胃癌发生中的作用提供了一项协议.
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