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相关概念视频

Epigenetic Regulation01:37

Epigenetic Regulation

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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
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相关实验视频

Updated: Jul 25, 2025

DNA Methylation: Bisulphite Modification and Analysis
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DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

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DNA甲基化分析分析

Naoko Hattori1, Yu-Yu Liu1, Toshikazu Ushijima2

  • 1Division of Epigenomics, Institute for Advanced Life Sciences, Hoshi University, Tokyo, Japan.

Methods in molecular biology (Clifton, N.J.)
|June 24, 2023
PubMed
概括
此摘要是机器生成的。

DNA甲基化分析揭示了关于细胞状态和疾病的关键信息. 提供了双硫酸盐转换,BeadChip阵列和定量甲基化特定PCR的协议,以准确检测DNA甲基化.

关键词:
双硫酸盐序列测序 双硫酸盐序列测序通过双硫酸盐介导的DNA转换.通过DNA甲基化.在Infinium中使用Infinium.甲基化特定的PCR进行甲基化.

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Targeted DNA Methylation Analysis by Next-generation Sequencing
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科学领域:

  • 表观遗传学和分子生物学
  • 基因组学和生物信息学

背景情况:

  • 基因甲基化调节基因表达,影响细胞功能和疾病状态.
  • DNA甲基化模式的改变对组织起源,疾病风险和患者预后具有信息意义.
  • 双硫酸盐转化是检测DNA甲基化的关键方法,尽管它有局限性.

研究的目的:

  • 为DNA甲基化分析提供全面的协议.
  • 详细介绍二硫酸盐转化方法和各种下游分析.
  • 为了能够准确检测和解释DNA甲基化状态.

主要方法:

  • 双硫酸转换DNA以区分甲基化和非甲基化细胞因子.
  • 使用BeadChip微阵列或下一代测序 (NGS) 进行全基因组分析.
  • 通过甲基化特异PCR (MSP) 和二硫酸盐测序进行区域特异分析.

主要成果:

  • 建立了双硫酸盐转换,Infinium BeadChip阵列,定量MSP和双硫酸盐测序的协议.
  • 证明了这些方法用于分析DNA甲基化的实用性.
  • 强调了精确的DNA甲基化检测对生物洞察的重要性.

结论:

  • 准确的DNA甲基化分析对于理解基因调节和疾病至关重要.
  • 提供的协议为全基因组和特定区域的DNA甲基化研究提供了可靠的方法.
  • 这些技术为各种应用程序的DNA甲基化数据的解释提供了便利.