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Cell Lines01:16

Cell Lines

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A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
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Lineage Commitment01:21

Lineage Commitment

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Commitment is the  process whereby stem cells:
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Updated: Jul 25, 2025

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
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HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL

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为特定的人类白细胞抗原呈现工程细胞系.

Dongbin Jin1, Khai Lee Loh2, Tima Shamekhi1

  • 1Department of Medicine, School of Clinical Sciences, Monash Univesity, Clayton, VIC, Australia.

Methods in molecular biology (Clifton, N.J.)
|June 24, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了一种方法来设计表达特定人类白细胞抗原 (HLA) 的细胞系,用于向免疫治疗研究. 这种技术有助于识别免疫性,对于开发有效的CD8+T细胞疗法至关重要.

关键词:
癌症 癌症 癌症 癌症细胞系 细胞系 细胞系基因编辑 基因编辑哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈免疫类药物 免疫类药物免疫治疗是一种免疫疗法.林氏病毒 (Lentivirus) 是一种病毒.新抗原是一种新抗原.转导 转导是指转导的过程.

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Generation of Human Alloantigen-specific T Cells from Peripheral Blood
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相关实验视频

Last Updated: Jul 25, 2025

HLA-Ig Based Artificial Antigen Presenting Cells for Efficient ex vivo Expansion of Human CTL
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科学领域:

  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学
  • 生物技术是生物技术.

背景情况:

  • 皮层特异性免疫疗法为癌症和自身免疫性疾病等疾病提供了向治疗.
  • 识别由人类白细胞抗原 (HLA) 类I呈现的免疫原对CD8+T细胞疗法至关重要.
  • 免疫组学是这种鉴定过程的关键技术.

研究的目的:

  • 提出一种新的方法,用于设计具有特定感兴趣的HLA的细胞系.
  • 为了能够从表达已定义HLA的细胞系中阐明免疫群.

主要方法:

  • 利用晶体病毒介导的转导来修改细胞系.
  • 采用细胞分类技术进行精确的基因工程.
  • 设计了一种细胞系以表达特定的HLA (HLA-A*02:01) 而缺乏本地HLA表达.

主要成果:

  • 成功创建了一种表达向人类白细胞抗原 (HLA) 的工程细胞系.
  • 证明了在工程细胞系中控制HLA表达的能力.
  • 建立了一个研究细胞系特异性免疫的平台.

结论:

  • 开发的方法为免疫学研究提供了一个强大的工具.
  • 这种技术有助于研究特定的HLA呈现及其在T细胞反应中的作用.
  • 能够推动针对性免疫疗法和诊断的发展.