环境对Streptococcus sanguinis膜囊泡生物发生的影响
Emily Helliwell1, Dongseok Choi2,3, Justin Merritt4,5
1Department of Restorative Dentistry, School of Dentistry, Oregon Health & Science University (OHSU), Portland, OR, USA. helliwel@ohsu.edu.
The ISME journal
|June 24, 2023
概括
细菌膜囊泡产生的Streptococcus sanguinis受其环境和邻居的影响. 这些囊泡与宿主细胞通信,触发免疫反应.
科学领域:
- 微生物学 微生物学
- 细菌生理学 细菌生理学
- 主体与微生物的相互作用
背景情况:
- 膜囊泡 (MVs) 对于细菌细胞之间的通信和宿主相互作用至关重要.
- 环境和社区对MV产生的影响,特别是像Streptococcus sanguinis这样的口腔初生的影响,尚不清楚.
研究的目的:
- 研究环境和社区因素对Streptococcus sanguinis膜囊泡产生的影响.
- 确定涉及S. sanguinis-宿主细胞通信的分子机制和囊泡载荷.
主要方法:
- 在不同共同培养条件下对S. sanguinis进行比较性转录组和蛋白组分析.
- 对各种口腔细菌的反应中MV生产 (数量和大小) 的分析.
- 在体外细胞培养试验中,使用牙上皮细胞来评估免疫反应.
主要成果:
- Porphyromonas gingivalis 消除了 S. sanguinis 的 MV 生产,而其他细菌没有显著的影响.
- 与细胞质膜相关的差异性基因表达和与MV丰富度相关的类甘油.
- S. sanguinis MVs诱导了在牙上皮细胞中显著表达促炎细胞因子 (IL-8,TNF-α,IL-1β,Gro-α).
结论:
- S. sanguinis 的 MV 生产受特定的细菌相互作用和环境线索的调节.
- 细菌MV在口腔细菌和宿主上皮细胞之间的沟通中起着重要作用,可能调节宿主免疫反应.
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