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Updated: Jul 25, 2025

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全基因组测序 Pseudomonas koreensis 从患病的Tor tambroides中分离出来的全基因组测序

Cindy Jia Yung Kho¹, Melinda Mei Lin Lau², Hung Hui Chung³

¹Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia. mariayung0617@gmail.com.

Current microbiology

|June 25, 2023

概括

一种新发现的鱼类病原体,Pseudomonas koreensis CM-01,通过全基因组测序来表征. 这种致病菌株具有独特的毒性因子和抗生素耐药性,使其与生活在土壤中的亲属有所区别.

科学领域:

微生物学微生物学
基因组学就是基因组学.
水生动物卫生水生动物卫生

背景情况:

Pseudomonas koreensis (P. koreensis) 已知在与土壤隔离时可以促进植物生长.
致病性P. koreensis菌株,特别是来自鱼类的菌株,报告不足.
Tor tambroides水产养殖受到未知的病原体的威胁.

研究的目的:

为了识别和描述致病的病原体,负责患病的Tor tambroides.
执行全基因组测序和分析新分离的P. koreensis菌株.
为了研究鱼类隔离的P. koreensis的致病性和毒性潜力.

主要方法:

从患病的鱼中分离和识别致病细菌.
孤立菌株 (CM-01) 的形态和生化特征.
全基因组测序,组装,注释和比较基因组分析.

主要成果:

一种新型致病性P. koreensis菌株CM-01被分离并进行了鉴定.
CM-01基因组 (6.17 Mbp,G+C 60.5%) 包含5538个蛋白质编码基因,毒性因子和抗生素耐药性基因.
CM-01对安皮西林,阿兹特雷诺姆,克林达米辛和塞福西丁具有耐药性,MAR指数为0.4;与其他P. koreensis菌株相比,发现了独特的致病性元素.

结论:

该研究报告了首次对来自鱼类的致病性P. koreensis菌株进行全面的基因组和表型表征.
已识别的毒性和抗生素耐药性基因为P. koreensis CM-01.1的病原性提供了洞察力.
这项研究为了解和管理水产养殖中的P. koreensis感染奠定了基础.

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