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纳米纤维聚烯/羊水膜通过促进巨细胞两极分化和调节炎症微环境,促进周围神经的再生

Chunjie Liu¹, Dengxiang Liu², Xiaochong Zhang³

¹Xingtai People's Hospital Postdoctoral Workstation, Xingtai People's Hospital, Xingtai 054031, China; Postdoctoral Mobile Station, Hebei Medical University, Shijiazhuang 050017, China; Department of Orthopedics, Tangshan Workers Hospital, Tangshan 063000, China.

International immunopharmacology

|June 25, 2023

概括

这项研究开发了一种聚卡普罗拉克/羊水膜 (PCL/AM) 纳米纤维复合材料,以改善神经修复. PCL/AM复合物促进M2巨细胞两极分化,减少炎症,并增强轴突再生,以改善神经愈合.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
神经科学是一个神经科学.

背景情况:

炎症在神经损伤的修复中起着双重作用,过度的炎症阻碍了神经的再生.
巨细胞,特别是M1和M2亚型,对于调节局部炎症环境至关重要.
有效的神经再生需要精确调节炎症反应和巨细胞两极分化.

研究的目的:

开发一种用于神经组织工程的多功能纳米纤维复合材料.
研究PCL/AM复合物的调节巨细胞极化和减少炎症的能力.
评估PCL/AM复合剂在促进神经再生和功能修复方面的有效性.

主要方法:

通过电制造使用多层多功能纳米纤维复合膜 (PCL) 和羊水膜 (AM).
在体外评估PCL/AM复合物对SH-SY5Y细胞轴突生长和神经元分化的影响.
在体内评估PCL/AM复合剂对神经微环境的影响,痕组织入侵,巨细胞招募和极化,炎症因子表达和神经再生.

主要成果:

在实验室中,PCL/AM复合物促进了SH-SY5Y细胞的轴突生长和神经元分化.
在体内,PCL/AM复合物形成了一个支持性的微环境,阻断了痕组织的入侵,并招募了巨细胞.
复合物诱导了M2巨分化,增强了抗炎因素 (IL-10,IL-13),抑制了促炎因素 (IL-6,TNF-α),并促进了髓和轴突再生.

结论:

PCL/AM纳米纤维复合物通过促进M2巨细胞两极分化和平衡炎症因素,有效调节局部炎症环境.
这种生物材料为增强轴突再生和改善神经修复结果提供了一个有希望的策略.
PCL/AM复合体显示出作为神经损伤治疗的治疗支架的巨大潜力.

关键词:

胚胎膜是一种氨基质膜.免疫调节免疫调节炎症炎症是一种炎症.巨细胞的两极分化外围神经的再生和再生.

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