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使用离心分离色谱学方法从蓝剩余中获得的蓝色安东素衍生物颜料的分离

Ana N Nunes¹, Viktoriya Ivasiv², Luís F Gouveia³

¹iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal; ITQB, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. Da República 2780-157 Oeiras, Portugal.

Journal of chromatography. A

|June 25, 2023

概括

这项研究介绍了一种可持续的离心分离色谱 (CPC) 方法,用于从蓝中净化天然的蓝色波提辛. 开发的CPC工艺比传统色谱更有效,更环保.

科学领域:

自然产品化学自然产品化学
染色学分离科学染色学分离科学
可持续的化学加工可持续的化学加工

背景情况:

越来越多的人对天然色素的需求超过了合成替代品.
自然存在的蓝色染料稀缺性是一个挑战.
农业副产品的价值化,比如蓝的剩余.

研究的目的:

开发一种高效和可持续的离心分离色谱 (CPC) 工艺,用于波提辛净化.
建立CPC作为逆相列染色学 (RP-CC) 的可行的替代方案,用于准备规模的隔离.
通过提取和净化天然蓝色染料来利用蓝的剩余价值.

主要方法:

从蓝的余中提取安托西亚宁和波提辛的半合成.
开发和优化用于CPC的双相溶剂系统.
评估CPC操作参数:样本负载,流量和旋转速度.
与逆相列色谱 (RP-CC) 进行CPC性能比较.

主要成果:

选择了一种两相溶剂系统 (三--甲基乙,n-butanol,acetonitrile,水,用HCl酸化).
在优化的CPC条件下,实现了5倍的波提辛净化.
与RP-CC相比,CPC的效率是2.4倍高,环境风险因子是5.5倍低.
成功地将蓝剩余的价值化为天然的蓝色色素.

结论:

离心分离色谱 (CPC) 是一种有效且可持续的方法,用于准备性分离波提辛.
与RP-CC相比,开发的CPC工艺在效率和环境影响方面提供了显著的优势.
这种方法可以从农业副产品中生产天然的蓝色染料.

关键词:

蓝残留物价值化蓝残留物价值化蓝色的颜料是一种蓝色的颜料.离心分区色谱学离心分区色谱学葡萄牙葡萄酒是一种葡萄酒.

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