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探索gliotoxin的抗菌作用:它是否会诱导氧化应激或蛋白质损伤?

Alexey S Vasilchenko¹, Elena V Gurina¹, Konstantin A Drozdov²

¹Laboratory of Antimicrobial Resistance, Institute of Ecological and Agricultural Biology (X-BIO), Tyumen State University, Tyumen, Russia.

|June 25, 2023

概括

Gliotoxin (GTX) 影响细菌的不同类型,导致氧化和热冲击压力. 它的抗菌作用被抗氧化剂和减少,但并没有完全阻断.

科学领域:

微生物学微生物学
菌类学菌类学是指菌类学.
生物化学生物化学

背景情况:

Gliotoxin (GTX) 是一种真菌代谢物,具有已知的真核细胞毒性.
目前尚不完全了解GTX的抗菌机制.
了解GTX的细菌相互作用对于潜在的治疗应用至关重要.

研究的目的:

为了研究 Gliotoxin 对 Gram 阳性和 Gram 阴性细菌的影响.
阐明GTX抗菌活性背后的机制.
为了确定影响GTX对细菌有效性的因素.

主要方法:

使用S. epidermidis和E. coli进行的比较吸收研究.
杀菌试验以确定有效的GTX剂量和时间框架.
生物发光生物传感器 (pKatG-lux,pIpbA-lux) 检测氧化和热冲击压力.
使用抗氧化剂 (Trolox,2-mercaptoethanol,减少的谷氨) 和盐进行预化.
SDS-PAGE用于分析蛋白质聚合.

主要成果:

格拉姆阴性细菌需要比格拉姆阳性细菌更高的GTX剂量才能在4小时内产生类似的杀菌效果.
在大肠杆菌中,GTX诱导了氧化应激 (pKatG-lux) 和在大肠杆菌中引起的热冲击应激 (pIpbA-lux).
2-甲基乙醇和减少的谷氨显著增加了GTX抑制剂量,而Trolox的影响最小.
在GTX治疗期间观察到蛋白质聚合.
添加盐显著抑制了GTX对细菌的作用.

结论:

GTX对格拉姆阳性和格拉姆阴性细菌表现出差异性影响,诱导特定的应激反应.
这些机制涉及氧化和热冲击压力,可能与蛋白质聚合有关.
抗氧化剂如2-乙醇和谷氨,尤其是,可以调节GTX的抗菌活性.

关键词:

抗生物膜活动活性.抗菌剂是一种抗菌剂.亚斯伯吉拉斯烟虫 (Aspergillus fumigatus) 是一种有毒的植物.埃皮波利提奥迪奥克索皮佩拉辛 (Epipolythiodioxopiperazine) 是一种可靠的药物.热冲击是一种热冲击.混合生物膜是一种混合生物膜.蛋白质聚合蛋白质的聚合.这就是ROSOS ROS.

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