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在日本的一名患者的胆汁中分离出cfr(B) 携带的Enterococcus raffinosus的完整基因组序列

Kasumi Ishida-Kuroki¹, Junzo Hisatsune², Takaya Segawa¹

¹Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Higashimurayama, Japan.

Journal of global antimicrobial resistance

|June 25, 2023

概括

一种携带线化物耐药基因cfr(B的新型Enterococcus raffinosus菌株被测序. 尽管存在耐药性基因,但细菌仍然对linezolid敏感,突出显示了移动耐药性基因的潜在储存库.

科学领域:

微生物学微生物学
基因组学就是基因组学.
抗微生物耐药性抗微生物耐药性

背景情况:

莱涅佐利德对于治疗VRE和MRSA等耐药细菌引起的感染至关重要.
在Enterococcus物种中出现的移动linezolid耐药基因是一个越来越令人担忧的问题.

研究的目的:

报告Enterococcus raffinosus JARB-HU0741.1. 的完整基因组序列.
在这种分离物中描述线化物耐药性基因cfr(B) 的存在和背景.

主要方法:

使用Illumina和牛津纳米孔技术进行全基因组测序.
使用Unicycler进行基因组组装,并使用DFAST进行注释.
使用Abricate和ResFinder数据库检测抗微生物耐药性基因.
通过汁微稀释来确定最小抑制度 (MIC).

主要成果:

拉菲诺斯 E. JARB-HU0741 的完整基因组包括一个染色体和一个巨质粒.
鉴定出cfr(B) 基因是在大质粒上的一种Tn6218类转子体内.
隔离物表现出对linezolid (MIC = 0.5μg/mL) 的敏感性,尽管它携带了cfr(B) 基因.

结论:

这是第一份关于E. raffinosus完整基因组的报告,其中包含cfr (B) 基因.
携带cfr(B) 的E. raffinosus没有表达的耐药性,作为移动耐药性基因的潜在储库,构成重大威胁.

关键词:

虫 (Enterococcus raffinosusus) 是一种可怕的细菌.基因组测序是指基因组的测序.线化物对线化物具有抗性.cfr (((B) 的情况.

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