长非编码RNA LTCONS4500通过向远鱼Miichthys miiuyuy中的miR-3570-5p促进了抗菌免疫反应

长非编码RNA LTCONS4500通过向远鱼Miichthys miiuyuy中的miR-3570-5p促进了抗菌免疫反应

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Qing Chu¹, Jingyao Yu¹, Jiale Zhou¹

¹School of Agriculture, Ludong University, Yantai, China.

Developmental and comparative immunology

|June 25, 2023

概括

一种新的长非编码RNA (lncRNA),LTCONS4500,通过调节炎症类细胞因子,增强了米尤克的免疫反应. 这种lncRNA作为竞争的内源RNA (ceRNA) 增强抗菌防御.

科学领域:

* 分子生物学 * 分子生物学
* 免疫学免疫学
* 水生动物健康

背景情况:

*长非编码RNAs (lncRNAs) 是生物过程中公认的基因调节者,包括先天免疫.
*与哺乳动物相比,远鱼免疫中的lncRNA-miRNA调节网络仍未得到充分探索.

研究的目的:

* 确定和描述 lncRNAs 在 miiuy croaker (Miichthys miiuy) 的免疫反应中的作用.
*阐明 lncRNAs 调节远鱼的抗菌免疫力的机制.

主要方法:

*研究了LTCONS4500在对细菌挑战 (Vibrio anguillarum,Vibrio harveyi) 的反应中的表达.
*分析了LTCONS4500对炎症性细胞因子表达的影响.
*使用竞争性内源RNA (ceRNA) 试验确定了LTCONS4500,miR-3570-5p和MyD88之间的相互作用.

主要成果:

* LTCONS4500的表达受到格拉姆阴性细菌的上调.
*高调的LTCONS4500促进了炎症性细胞因子的表达.
* LTCONS4500作为miR-3570-5p的ceRNA功能,增强MyD88表达和抗菌免疫力.

结论:

* LTCONS4500是米克罗克的抗菌免疫反应的积极调节者.
* LTCONS4500/miR-3570-5p/MyD88轴是远鱼天生的免疫的一个关键机制.
*这些发现有助于理解脊椎动物免疫中的lncRNA介导的基因调节.

关键词:

抗菌性免疫反应抗菌性免疫反应长长的非编码RNAs.我的D8888是什么意思远距离鱼是一种鱼类.ceRNARNA是什么意思

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