RNA结合蛋白ZC3H11A与核多A结合蛋白PABPN1相互作用,并改变病毒转录的多化
Katharina Kases1, Erik Schubert1, Zamaneh Hajikhezri1
1Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
The Journal of biological chemistry
|June 25, 2023
概括
RNA结合蛋白ZC3H11A与PABPN1和病毒mRNA相互作用,调节核mRNA的多化. 这项研究揭示了ZC3H11AA.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 生物化学 生物化学
背景情况:
- 核mRNA代谢涉及蛋白质调节和复合物形成.
- ZC3H11A是一种RNA结合蛋白,对mRNA输出,信号传递和发育至关重要.
- 已知ZC3H11A在核复制病毒中的作用,但生化细节很少.
研究的目的:
- 为了生物化学地表征ZC3H11A蛋白质.
- 确定ZC3H11A的相互作用伙伴,并阐明其在核mRNA代谢中的功能.
- 为了研究ZC3H11A在病毒mRNA处理中的作用.
主要方法:
- 蛋白质组学:在人类和小鼠细胞中建立了ZC3H11A蛋白相互作用体.
- 生物化学分析:证明了ZC3H11A,PABPN1和病毒mRNA之间的特定相互作用.
- 功能性测试:评估ZC3H11A对病毒mRNA多基解的影响.
主要成果:
- 确定了PABPN1作为ZC3H11A的特定相互作用者,控制其核斑点定位.
- 显示的ZC3H11A通过共享的指图案与人类腺病毒5型 (HAdV-5) 囊mRNA相互作用,这取决于PABPN1.
- 证明ZC3H11A缺陷改变了HAdV-5体mRNA多基化.
结论:
- ZC3H11A使用保存结构动机与PABPN1和病毒mRNA相互作用.
- ZC3H11A在调节核病毒mRNA的多基解中起到PABPN1依赖的作用.
- 而ZC3H11A则成为核mRNA多基解的潜在新型调节剂.
相关概念视频
Leaky Scanning
5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K
RNA Editing
9.1K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.1K
RNA Interference
26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K
Chromatin Structure Regulates pre-mRNA Processing
7.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.1K
RNA Polymerase II Accessory Proteins
9.3K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.3K
Nuclear Export of mRNA
7.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.7K


