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储存对酸处理的血小板的质量和功能的影响,其 HLA I 类表面表达减少

Mohammad Reza Mirlashari¹, Annette Vetlesen¹, Lise Sofie H Nissen-Meyer¹

¹Department of Immunology and Transfusion Medicine, Oslo University Hospital, Oslo, Norway.

Transfusion medicine (Oxford, England)

|June 25, 2023

概括

酸性治疗减少了血小板上的HLA类I复合体,为耐火患者提供了替代方案. 这些经过修改的血小板可以维持长达3天的功能,从而提高了输血的灵活性.

科学领域:

血液学血液学血液学
免疫学免疫学免疫学
输血医学输血医学

背景情况:

耐药患者需要与HLA匹配的血小板,需要耗时的交叉匹配.
血小板 (PLT) 用酸变质HLA I类复合物的处理,保持PLT功能.
降低HLA I级的血小板提供了与HLA匹配的PLT进行输血的替代方案.

研究的目的:

评估酸治疗在从血小板中消耗HLA类I复合物的有效性.
在储存期间评估酸处理血小板的活力,激活和功能.
为了确定用于输血的酸处理血小板的延长存储潜力.

主要方法:

布菲外套血小板缩剂用酸处理,以耗尽HLA I类复合物.
流细胞计评估了HLA类I,CD62P和CD63的表面表达,以及活力和线粒体膜潜力 (MMP).
血小板结晶学 (TEG) 评估了血小板的功能.

主要成果:

酸性治疗从第1天到第7天减少了70%-75%的HLA I类表达.
在对照和酸处理血小板中,活力和MMP在长达4天内保持稳定.
血小板激活潜力和活力在7天后显著下降.
酸性治疗显著减少了TEG反应时间 (R时间) 从第1天到第3天.

结论:

用酸处理的血小板可以长时间储存,提高了输血计划的灵活性.
这种方法为因抗HLA抗体而导致异免疫血小板折射症的患者提供了可行的替代方案.

关键词:

HLA 一级的第一类.免疫性血小板减少症.血小板输血输血血小板输血血小板血小板是

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