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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jul 25, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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nASAP:一个新兴的RNA分析数据分析平台.

Zhi Wang1, Peng Ge1, Xiao-Long Zhou1

  • 1State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.

Journal of molecular biology
|June 25, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了nASAP,这是一个用户友好的网络服务器,用于分析新生的RNA测序数据. 它提供了必要的质量控制和全面分析,改进了转录调节研究.

关键词:
基因表达的基因表达方式基因监管网络 基因监管网络新兴的RNA分析暂停暂停暂停的时间质量评估质量评估的质量评估.网络服务器是Web服务器.

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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 新生的RNA测序对于研究转录调节至关重要.
  • 对于新兴RNA的现有数据处理管道缺乏标准化和全面的分析工具.
  • 对于新兴的RNA数据,需要可访问和强大的生物信息解决方案.

研究的目的:

  • 推出nASAP,这是一个新的Web服务器,旨在对新兴RNA数据集进行质量评估和全面分析.
  • 解决用于新兴RNA分析的标准化数据处理管道的缺口.
  • 通过先进的数据分析,促进对基因调节机制的更深入的理解.

主要方法:

  • 开发了nASAP网络服务器 (https://grobase.top/nasap/).
  • 实施了四个定制的分析模块:质量评估,量化分析 (mRNAs, lncRNAs, eRNAs),全基因组暂停分析和网络分析 (增强剂-促进剂相互作用体).
  • 使用测序阅读分布进行暂停分析和注释互动组进行网络分析.

主要成果:

  • nASAP提供实用的质量评估,包括测序统计,映射比率,RNA完整性和mRNA污染评估.
  • 服务器允许对mRNA,lncRNA和eRNA进行量化.
  • 综合了全基因组暂停分析和基因调节洞察力的网络分析.
  • 与现有管道相比,nASAP在质量控制方面表现优越.

结论:

  • nASAP提供了一个用户友好的平台,用于新兴RNA数据的基本和高级分析.
  • 网络服务器标准化和增强新兴RNA分析的分析,填补了该领域的关键空白.
  • 预计nASAP将在研究转录调节的各种研究领域具有高度价值.