通过功能不明的蛋白质LysV,通过蛋白质与蛋白质相互作用调节Thermus thermophilus的氨酸生物合成
Yutaro Morita1, Ayako Yoshida1,2, Siyan Ye1
1Graduate School of Agricultural and Life Sciences, The University of Tokyo.
The Journal of general and applied microbiology
|June 26, 2023
概括
在Thermus thermophilus中素的生物合成涉及蛋白质LysW. 一种相关的蛋白质LysV与生物合成酶相互作用,这表明它通过蛋白质与蛋白质相互作用来调节这一关键的代谢途径.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 热热通过α-aminoadipate通路合成氨酸.
- 氨基基组载体蛋白质LysW对于将中间体转移到生物合成酶至关重要.
- 一种假设的蛋白质LysV与LysW有相似之处,位于lysine生物合成基因集群中.
研究的目的:
- 为了研究假设蛋白质LysV在Thermusthermophilus lysine生物合成中的功能作用.
- 为了确定LysV是否与已知的lysine生物合成酶相互作用.
- 阐明 LysV 可能影响 lysine 生产的机制.
主要方法:
- 基因淘汰实验以评估lysV的必要性.
- 拉下测试用于检测蛋白质与蛋白质相互作用.
- 交叉连接实验以确认LysV与生物合成酶的相互作用.
- 酶活性测试用于评估LysV对催化功能的影响.
主要成果:
- 莱斯V淘汰并没有导致氨酸辅,这表明在测试条件下,它对生存并不必不可少.
- 发现LysV与正常使用LysW的所有测试生物合成酶相互作用.
- 与LysV相互作用的大多数酶的活性受到其存在的显著影响.
结论:
- 在Thermus thermophilus lysine生物合成中,lysV起着调节性的作用.
- LysV 可能通过与关键生物合成酶的直接蛋白质-蛋白质相互作用影响氨酸的产生.
- lysV同类的保存性表明,相关微生物中存在着保存的调节机制.
更多相关视频
12:49Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
Published on: April 4, 2018
11.7K
07:02Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
Published on: March 11, 2012
13.4K
相关概念视频
Covalently Linked Protein Regulators
6.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.9K
Transcription Attenuation in Prokaryotes
15.6K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.6K
Prokaryotic Transcriptional Activators and Repressors
21.2K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.2K
Regulated Protein Degradation
7.4K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.4K
Translational Regulation
45
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
45
Regulation of Expression at Multiple Steps
951
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
951
