Circ_0000033通过隔离miR-378a-3p对NUAK2进行上调,以促进乳腺瘤的发生
Yijun Dai1, Wenjian Shi2, Yanru Qiu1
1Department of Medical Oncology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.
Environmental and molecular mutagenesis
|June 26, 2023
概括
循环RNAs (circRNAs),特别是circ_0000033,在乳腺癌 (BC) 中表达高,并通过调节miR-378a-3p/NUAK2通路来促进瘤生长. 沉默circ_0000033抑制了BC的进展,突出了其作为治疗目标的潜力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 循环RNAs (circRNAs) 越来越多地被认为是它们在癌症发展中的作用.
- 在乳腺癌 (BC) 中观察到circ_0000033的异常表达,这表明它具有潜在的调节功能.
研究的目的:
- 研究circ_0000033在乳腺癌 (BC) 致癌的作用和潜在机制.
- 为了阐明涉及circ_0000033,miR-378a-3p和BC的NUAK2的监管网络.
主要方法:
- 使用定量实时PCR (qRT-PCR) 和西部抹杀来分析基因和蛋白质表达.
- 在体外测试 (殖民地形成,EDU,流细胞计,caspase-3活性,transwell,管形成) 和体内小鼠异种移植模型被用于评估细胞和瘤行为.
- 双露西法酶记者测定验证了circ_0000033,miR-378a-3p和NUAK2.2之间的结合相互作用.
主要成果:
- 在BC组织和细胞中,Circ_0000033被显著上调.
- 敲除circ_0000033抑制了BC细胞的增殖,迁移,入侵,血管生成,诱导了细胞亡和细胞循环停止.
- Circ_0000033作为miR-378a-3p的分子海绵,导致NUAK2的表达增加并促进乳腺瘤发生.
结论:
- 通过对miR-378a-3p进行隔离,Circ_0000033通过上调NUAK2促进乳腺癌的进展.
- 该circ_0000033/miR-378a-3p/NUAK2轴代表了BC的一个新的调节途径.
- Circ_0000033是乳腺癌治疗的潜在治疗标.
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