相关实验视频

Updated: Jul 25, 2025

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition

Published on: June 10, 2016

来自中细胞的细胞外囊包装ILK促进腹膜纤维化中的纤维细胞激活

Qiang Huang¹, Yuxiang Sun¹, Long Peng²

¹Nephrology Division, Department of Medicine, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Journal of extracellular vesicles

|June 26, 2023

概括

腹腔透析患者的细胞外囊泡 (EVs) 通过转移整合素相关激酶 (ILK) 来促进纤维化. 阻断EV或ILK可以治疗腹膜纤维化并改善透析功能.

科学领域:

腎臟病學 (nephrology) 是一種醫學專業.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

长期腹膜透析 (PD) 可能导致腹膜纤维化和功能丧失,导致PD治疗失败.
腹膜细胞之间的细胞间通信在腹膜纤维化中至关重要.
细胞外囊泡 (EVs) 调解细胞-细胞通信,但它们在腹膜纤维化中的作用尚不清楚.

研究的目的:

调查长期PD期间腹膜纤维化中EVs的作用和机制.
确定潜在的治疗点,以预防PD相关的腹纤维化.

主要方法:

从PD患者的废水中对EV蛋白质组学和单细胞RNA测序进行综合分析.
用转化生长因子-β1 (TGF-β1) 刺激介质细胞以诱导EV释放.
使用GW4869或Rab27a敲击抑制EV分泌.
在纤维细胞中分析整合素结合激酶 (ILK) 表达及其信号通路 (p38 MAPK).
在PD患者中,ILK阳性EV与腹功能障碍的临床相关性.

主要成果:

中皮细胞是PD废水中EVs的主要来源.
由TGF-β1刺激的介质细胞衍生的EVs促进纤维细胞激活和腹膜纤维生成.
阻止EV分泌显著减少PD诱导的纤维细胞激活和纤维化.
由PD衍生的EV含有高水平的ILK,该ILK通过p38 MAPK通路激活纤维细胞.
在纤维化腹膜中ILK表达升高,并且与PD患者的腹膜功能障碍相关.

结论:

腹腔内膜细胞介导介质细胞和纤维细胞之间的通信,启动腹腔内膜纤维化.
准EV或ILK为PD患者腹膜纤维化提供了一个新的治疗策略.

关键词:

这是一个很好的方法. ILK ILK 细胞与细胞之间的通信.细胞外囊泡中的细胞外囊泡.腹膜透析是指腹膜透析.腹膜纤维化腹膜纤维化腹膜体内膜是什么腹膜体内膜

更多相关视频

In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis

In vitro Mesothelial Clearance Assay that Models the Early Steps of Ovarian Cancer Metastasis

Published on: February 17, 2012

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

Published on: June 27, 2017

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...

Extracellular Matrix

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...