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使用伴侣蛋白Spy作为Bacillus subtilis中的融合标签,有效分泌贝粘附蛋白

Panpan Wu¹, Qing Tao¹, Yuxuan Liu¹

¹Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, People's Republic of China.

Biotechnology journal

|June 26, 2023

概括

研究人员成功地通过Bacillus subtilis的融合标签策略分泌了脚蛋白 (Mfps). 这一突破使得这些强大的粘合蛋白能够大规模生产,用于各种应用.

科学领域:

生物技术是生物技术.
蛋白质工程是指蛋白质工程.
微生物表达系统微生物表达系统

背景情况:

贝类脚蛋白 (Mfps) 具有显著的粘合性.
重组表达是大规模Mfp合成的关键.
在异质宿主中Mfps的分泌表达仍然是一个挑战.

研究的目的:

为了在 Bacillus subtilis 中实现 Mfp3 和 Mfp5 的高效分泌表达.
开发一种可扩展的方法来生产Mfps.

主要方法:

在B. subtilis.中选Mfp分泌的信号.
使用伴侣蛋白Spy作为Mfp分泌的融合标签.
使用TEV蛋白酶裂变和NTA亲属性染色学净化Mfp3和Mfp5.

主要成果:

在B. subtilis中,伴侣蛋白Spy被有效分泌出来.
融合蛋白Spy-Mfp3-6×His和Spy-Mfp5-6×His成功地被分泌出来.
在瓶条件下,Spy-Mfp3-6×His (255 mg/L) 和Spy-Mfp5-6×His (119 mg/L) 的高产量得到了实现.

结论:

在B. subtilis中,Mfp3-6×His和Mfp5-6×His可以有效地分泌.
与伴侣蛋白Spy的融合增强了Mfp的分泌.
这种方法促进了Mfps的大规模生产.

关键词:

B. subtilis 的意思,就是说.间间是什么意思间间的粘附蛋白质的粘附蛋白质秘密表达的表达方式

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