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菌诱导性染色体群岛作为捕获和检测细菌病原体的诊断平台

Rodrigo Ibarra-Chávez^1,2,3, Julien Reboud³, José R Penadés^2,4,5

¹Department of Biology, Section of Microbiology, University of Copenhagen, Universitetsparken 15, bldg. 1, Copenhagen, DK2100, Denmark.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|June 26, 2023

概括

菌体诱导性染色体岛屿 (PICI) 被重新设计为新型诊断工具. 该系统能够灵敏地视觉检测可活性的细菌,如大肠杆菌和黄金杆菌,其性能优于传统方法.

科学领域:

合成生物学合成生物学
微生物学微生物学
生物技术是生物技术.

背景情况:

菌体诱导性染色体岛屿 (PICI) 是可移动的遗传元素,它们利用菌体机械进行传播.
目前正在探索PICI的新生物技术应用,包括细菌检测.

研究的目的:

开发一个用户友好的诊断系统,利用PICI转导进行选择性细菌丰富和检测.
为了证明该系统对特定细菌物种,大肠杆菌和金黄色葡萄球菌的顺序恢复的能力.

主要方法:

利用PICI转导来选择性丰富目标细菌.
实施一个记者基因系统,用于可行的病原体的视觉感知.
将基于PICI的检测灵敏度和特异性与基于菌体的传统方法进行比较.

主要成果:

实现了高传输速率和细菌检测的灵敏度,降至大约50 CFU mL-1.1.
启用可视感知活力的细菌,区分它们与死细胞.
证明了大肠杆菌和金黄色杆菌的连续恢复.

结论:

基于PICI的诊断系统提供了一种敏感和特定的方法来检测可活性的细菌.
这种方法提供了一个视觉,活细胞特定的检测机制,超越了基于核酸的测试的局限性.
开发的系统扩展了合成生物学和生物传感应用的工具包.

关键词:

在PICI中,PICI是PICI.细菌检测检测细菌的检测诊断诊断诊断诊断诊断移动的遗传元素是移动的.纸张的微流体学菌体卫星菌体卫星

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