优化简单的一步便处理方法来诊断结核病:对便的稳固性和运输条件的评估,用于全球实施
Bazezew Yenew1, Petra de Haas2, Getu Diriba1
1Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Microbiology spectrum
|June 26, 2023
概括
简单的一步便处理方法是有效的诊断结核病 (TB) 在儿童. 微小的协议更改是可以接受的,但最佳的运输和样本数量是准确结果的关键.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 儿科 儿科 儿科
背景情况:
- 对于需要使用替代方法来诊断结核病 (TB) 的幼儿来说,液采集具有挑战性.
- 简单的一步 (SOS) 便处理方法为使用Xpert MTB/RIF Ultra (Xpert-Ultra) 检测Mycobacterium结核病 (MTB) 提供了一种新的方法.
研究的目的:
- 评估SOS便处理方法的稳定性,并确定用于结核病诊断的最佳便样本运输条件.
- 评估运输时间,温度和加工步骤的变化对Xpert-Ultra测试结果的影响.
主要方法:
- 模拟运输条件被用来测试结核病确诊参与者的便样本.
- 便处理遵循标准的SOS协议,但稍有修改,并进行Xpert-Ultra测试.
- 总共分析了47名结核病参与者的132个便样本中的2,963个Xpert-Ultra结果.
主要成果:
- 与SOS协议的轻微偏差没有显著影响Xpert-Ultra测试结果.
- 非冷链运输,长时间暴露在室温 (>12h) 和过量的便量 (>0.8g) 增加了Xpert-Ultra处理错误.
- 建议调整便量范围 (0.3至0.8g) 以获得最佳结果.
结论:
- 该SOS便处理方法是强大的结核病诊断在儿童中,与小的协议灵活性.
- 最佳的便运输条件和样本数量对于可靠的Xpert-Ultra检测至关重要.
- 调整SOS方法,特别是样本量,可以促进在资源有限的环境中扩大基于便的结核病诊断.
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