在克拉米迪亚的III型分泌物
1Department of Pathology and Microbiology, University of Nebraska Medical Center, Durham Research Center II, Omaha, Nebraska, USA.
Microbiology and molecular biology reviews : MMBR
|June 26, 2023
概括
第三类分泌系统 (T3SSs) 对于Chlamydiaceae的致病性至关重要,它们提供作用因子来操纵宿主细胞. 本综述详细介绍了T3SS在整个克拉米迪亚发育周期中的发现,功能和作用因子.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病原发生和发病.
背景情况:
- 第三类分泌系统 (T3SS) 是格拉姆阴性病原体中必不可少的毒性因子,可直接注射效应蛋白到宿主细胞中.
- 克拉米迪亚科,有义务的细胞内细菌,具有高度保存的T3SS,对它们的生存和繁殖至关重要,它们的基因组中有很大一部分用于T3SS组件.
- 甲状腺菌表现出双相发育周期 (传染性基本体和复制性网状体),T3SS结构和效应器在不同阶段活跃.
研究的目的:
- 审查克拉米迪亚T3SS装置及其相关伴侣的历史发现和生物化学表征.
- 阐明T3SS在整个克拉米迪亚发育周期中的功能作用.
- 讨论最近在理解甲状腺菌效应因子方面的进展,以及在T3SS研究中使用替代模型.
主要方法:
- 审查现有的关于在Chlamydiaceae中发现和表征T3SS的文献.
- 对T3SS设备组件和伴侣的生物化学分析.
- 在克拉米迪亚发育周期的不同阶段对T3SS效应物的功能分析.
- 使用异质/代用模型研究克拉米迪亚T3SS.
主要成果:
- 克拉米迪亚T3SS是一种保存的,对于致病的重要系统,在整个生物体的两相生命周期中运行.
- 生物化学表征已经确定了T3SS装置和陪伴者的关键组件,尽管遗传工具的局限性.
- 由T3SS传递的效应蛋白在克拉米迪亚进入,细胞内存活和退出中发挥作用.
结论:
- 克拉米迪亚T3SS是一种中央毒性决定因素,是克拉米迪亚科独特生物学和病变发生的组成部分.
- 了解T3SS及其效应器可以了解宿主-病原体相互作用和潜在的治疗点.
- 异质系统是剖析克拉米迪亚T3SS的复杂机制的宝贵工具.
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