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快速和视觉检测特定细菌的唾液和阴道液识别与侧向流量测量棒策略

Daijing Yu^1,2, Jun Zhang^1,2, Niu Gao^1,2

¹School of Forensic Medicine, Shanxi Medical University, Jinzhong, 030600, Shanxi, People's Republic of China.

International journal of legal medicine

|June 26, 2023

概括

一种新的聚合酶连锁反应 (PCR) 和侧流滴棒 (LFD) 测定方法使用微生物DNA快速识别唾液和阴道液. 这种方法对于法医分析来说是简单,可靠和高效的.

科学领域:

法医科学法医科学法医科学
微生物学微生物学
分子生物学分子生物学

背景情况:

准确识别体液对于犯罪现场调查至关重要.
目前的微生物DNA分析方法 (测序,qPCR) 是复杂的,耗时的和昂贵的.
需要快速,简单,经济高效的体液识别技术.

研究的目的:

开发和验证一种新型的聚合酶连锁反应 (PCR) 结合侧向流水棒 (LFD) 试验,用于同时检测Streptococcus salivarius (在唾液中) 和Lactobacillus crispatus (在阴道液中).
在模拟法医样本中评估测试的灵敏度,特异性和性能.
评估已识别的DNA与下游短并列重复 (STR) 分析的兼容性.

主要方法:

开发一种多重PCR试验,针对唾液 (S. salivarius) 和阴道液 (L. crispatus) 的特定微生物DNA标记物.
将PCR测定与侧向流量杆 (LFD) 集成,以快速视觉检测.
在各种体液 (唾液,阴道液,血液,精液,鼻液,皮肤) 和具有不同DNA混合比率的模拟法医样本上测试PCR-LFD测定.

主要成果:

通过PCR-LFD测定,通过分别检测S. salivarius和L. crispatus,成功并具体识别了唾液和阴道液.
通过0.001 ng/μL DNA的检测极限实现了高灵敏度,可在3分钟内观察到.
该测试证明了稳定性,即使在1:999的DNA混合比率下也准确地识别了目标液体,并且显示了其他测试的体液的负结果.
用于体液识别的DNA样本也产生了完整的STR概况.

结论:

开发的PCR-LFD试验提供了一种快速,简单,可靠和高效的方法,用于识别法医样本中的唾液和阴道液.
这种技术克服了现有方法的局限性,为法医调查提供了具有成本效益的解决方案.
该试验能够提供适合STR分析的DNA,为全面的法医分析增加了显著的价值.

关键词:

牛乳杆菌克里斯帕图斯 (Lactobacillus crispatus) 是一种细菌.侧向流量测量棒横向流量测量棒这是唾液的唾液.这是一种Streptococcus salivarius.阴道液体阴道液体

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