代谢产生的进化相关性与海洋海绵细菌共生体的关系
Alshaimaa M Hamoda1,2,3, Rania Hamdy1,4, Bahgat Fayed1,5
1Research Institute for Medical and Health Sciences, University of Sharjah, P.O. Box 27272, Sharjah, UAE.
Applied microbiology and biotechnology
|June 26, 2023
概括
海洋海绵细菌产生的化合物对真菌和哺乳动物细胞有选择性的毒性,而不是细菌. 这种化合物M1及其水解产物DTBPA具有强烈的抗真菌活性,这表明它具有针对海绵病原体的新型防御机制.
科学领域:
- 海洋微生物学 海洋微生物学
- 自然产品化学 自然产品化学
- 共生相互作用的共生相互作用.
背景情况:
- 海绵是各种微生物群落的宿主,形成了微生物提供防御的共生关系.
- 通常在海洋海绵中发现的细菌物种是新生物活性化合物的潜在来源.
研究的目的:
- 从海洋海绵中分离和描述共生细菌.
- 在优化的发酵条件下研究分离细菌的代谢特征.
- 识别和评估细菌产生的特定化合物的生物活性.
主要方法:
- 隔离和培养Bacillus spp.的丰富. 来自一个海洋海绵.
- 使用薄层染色学 (TLC) 和气体染色学-质谱学 (GC-MS) 的代谢分析.
- 大规模发酵,化合物分离和结构识别 (M1和DTBPA).
- 对原生细胞和真核细胞进行抗微生物和细胞毒性查.
主要成果:
- 细菌种类. 细菌种类. 从海洋海绵中分离出来的.
- 优化的发酵条件 (海洋模拟营养和温度) 产生了各种代谢物.
- 化合物M1 (octadecyl-1-(2',6'-di-tert-butyl-1'-hydroxyphenyl) 酸盐) 对真核细胞 (真菌和哺乳动物细胞) 具有选择性毒性,但对 prokaryotic 细菌没有.
- M1的水解产物DTBPA显著增强了抗真菌活性.
结论:
- 细菌种类. 细菌种类. 产生一种选择性有毒的化合物 (M1),作为一种潜在的防御性代谢物对抗真核病原体.
- 代谢辅助发酵对于从海洋共生生物中发现新的生物活性化合物是有效的.
- 这些发现突显出一种独特的海洋进化防御策略,涉及共生细菌及其代谢物.
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