用于检测核酸和非核酸目标的CRISPR/Cas系统
Weiran Su1,2,3, Junru Li1,2,3, Chen Ji1,2,3
1State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200032 China.
Nano research
|June 26, 2023
概括
集群定期间隔的短平行体重复 (CRISPR) /与CRISPR相关的 (Cas) 系统提供了多功能生物传感能力. 本综述详细介绍了CRISPR/Cas用于检测各种目标的应用,强调了它们在疾病生物标志物检测方面的潜力和挑战.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 生物感应是一种生物感应.
背景情况:
- 越来越多的CRISPR/Cas系统被用于检测疾病生物标志物.
- 这些系统表现出特定的识别, cis-cleavage 和非特定的 trans-cleavage 活动.
- 应用范围扩展到核酸 (DNA,RNA) 和非核酸点 (蛋白质,外体,细胞,小分子).
研究的目的:
- 审查各种CRISPR/Cas系统的原则和特征.
- 突出CRISPR/Cas系统在目标检测中的多样化应用.
- 讨论CRISPR/Cas系统在生物传感中的前景和挑战.
主要方法:
- 对CRISPR/Cas系统的审查,包括CRISPR/Cas9,Cas12,Cas13和Cas14.
- 收集用于检测核酸和非核酸目标的应用程序.
- 对基于CRISPR/Cas的生物传感的当前文献进行分析.
主要成果:
- 克里斯普尔/卡斯系统在检测广泛的目标方面具有广泛的适用性.
- 特定的CRISPR/Cas变体 (Cas9,12,13,14) 为不同的应用提供了明显的优势.
- 该审查对许多成功的检测策略进行了分类和详细说明.
结论:
- 克里斯普尔/卡斯系统是生物传感和疾病生物标志物检测的强大且可适应的工具.
- 需要进一步的研究来克服挑战,并充分实现CRISPR/Cas在临床应用中的潜力.
- 由于CRISPR/Cas系统的多功能性,预示着诊断技术的重大进步.
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