光谱光处理影响了印度树的形态生理性质和卡瓦克罗尔积累
Waikhom Bimolata1, Raktim Bhattacharya1, Ambika Goswami1
1Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721302 India.
概括
红蓝色LED灯显著增强印度的植物化学物质. 这种光谱光能促进生长,卡瓦克罗尔和抗氧化活性,使其非常适合医用草药种植.
科学领域:
- 植物科学 植物科学
- 农业工程 农业工程
- 生物化学 生物化学
背景情况:
- 发光二极管 (LED) 提供增强的植物质量.
- 印度 (Plectranthus amboinicus) 是一种富含卡瓦克罗尔的药用草本.
- 关于光谱光对VOC和P. amboinicus基因表达的影响的数据有限.
研究的目的:
- 研究P. amboinicus对不同LED光谱光的形态生理学,生物化学和转录反应.
- 确定最佳的LED光谱,以最大限度地提高植物化学物质的积累,特别是卡瓦克罗尔.
- 分析光谱光对类生物合成基因表达的影响.
主要方法:
- 植物被用红色,绿色,蓝色,温暖的白色和红蓝色 (1:1) LED 照射在 40 ± 5 μmol m−2 s−1 处,持续 40 天.
- 评估的生长指数,叶子的新鲜和干燥重量,素含量和抗氧化活性.
- 量化了卡瓦克罗尔的积累,并分析了关键类生物合成基因 (PaDXS,PaDXR,PaHMGR,PaCYP1,PaCYP9) 的表达.
主要成果:
- 红蓝 (1:1) LED处理产生了最大的生长指数,叶子重量和卡瓦克罗尔积累 (14.45 μmol g-1 FW).
- 与温暖的白色LED相比,醇含量增加了一倍,抗氧化活性增加了2.5倍.
- 在红蓝 (1:1) 光线下,在腺体三体中观察到高和化合物的沉积.
- 在红蓝色 (1:1) 和绿色光条件下,类生物合成基因显著上调.
结论:
- 红蓝 (1:1) LED光线是最大限度地提高P. amboinicus中的植物化学物质的最有效的光谱选项.
- 光谱光显著影响植物生长,卡瓦克罗尔含量和基因表达.
- 进一步的研究将探索最佳的红色和蓝色LED比率,以增强植物化学积累.
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