与IgG1的正确基因相比,Glyco设计的SARS-CoV-2IgM呈现出更高的活性
Somanath Kallolimath1, Roman Palt1, Esther Föderl-Höbenreich2
1Institute of Plant Biotechnology and Cell Biology, Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.
Frontiers in immunology
|June 26, 2023
概括
糖基工程植物成功地产生了针对SARS-CoV-2的pentameric免疫球蛋白M (IgM) 抗体. 与IgG对应物相比,这些植物性IgM显著增强了抗原结合和病毒中和.
科学领域:
- 生物技术是生物技术.
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 免疫球蛋白M (IgM) 是最大的抗体同型,对免疫反应至关重要,但难以产生以及定义良好的多分子.
- 鉴定IgM的独特特性,如广泛的糖化和寡合化,受到生产困难的阻碍.
- 感染SARS-CoV-2 (严重急性呼吸系统综合征冠状病毒2) 需要有效的基于抗体的诊断和治疗.
研究的目的:
- 在糖基工程植物中表达SARS-CoV-2中和单克隆抗体作为功能性免疫球蛋白M (IgM).
- 研究同型从IgG1转换为IgM对抗体特性和疗效的影响.
- 展示基于植物的系统在生产具有特定翻译后修改的复杂人体蛋白质方面的潜力.
主要方法:
- 利用糖基工程植物来表达两种SARS-CoV-2中和单克隆抗体.
- 从父母IgG1转换成IgM格式进行同型切换.
- 分析了重组IgMs的组合,糖化概况,抗原结合和病毒中和功效.
主要成果:
- 成功产生了由21个人类蛋白质子单元组成的米克免疫球蛋白M (IgM) 抗体.
- 实现了高度可重复的,人类类型的N-糖化形状,每个甘油酸在每个甘油酸中只有一个主导的N-糖类物种.
- 与父母的IgG1抗体相比,Pentamic IgM的抗原结合和病毒中和能力提高了多达390倍.
结论:
- 糖基工程植物是生产复杂,功能性免疫球蛋白M (IgM) 抗体的多功能平台,具有向的糖基化.
- 来自植物的Pentameric IgM表现出优越的抗原结合和中和功效,比IgG格式具有优势.
- 这些发现对开发下一代疫苗,诊断和基于抗体的疗法有重大影响.
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