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基于RNA-Seq的基因变异的发现和两层线和肉的等位基因特异性表达

Muhammad Arsalan Iqbal¹, Frieder Hadlich¹, Henry Reyer¹

¹Research Institute for Farm Animal Biology Institute of Genome Biology Dummerstorf Germany.

Evolutionary applications

|June 26, 2023

概括

这项研究使用RNA测序来分析肉和之间的遗传多样性,识别与经济重要特征相关的基因. 层中的等位基特异性表达揭示了它们的寿命期间的代谢适应,特别是在产卵高峰期.

科学领域:

家禽遗传学家禽遗传学
文字转录学 (Transcriptomics) 是一个学科.
人口多样性人口多样性

背景情况:

选择性繁殖迅速推进了家禽生产,创造了不同的肉和层种群.
了解这些群体之间的遗传和功能多样性对于进一步的行业发展至关重要.

研究的目的:

使用转录组变异调用来确定 brojler 和层之间的种群多样性.
为了确定与家禽经济上重要的特征相关的候选基因.
为了研究一代基因特异性表达 (ASE) 模式在整个生命周期的层,并将它们与代谢过程联系起来.

主要方法:

分析了来自肉和两层品种的200个个人的RNA测序数据.
进行变量调用和固定指数 (FST) 分析以评估人口差异化.
在多个年龄段的层菌株的肠道粘膜中进行了基因特异性表达 (ASE) 分析.

主要成果:

识别了许多与生长,新陈代谢和免疫相关的候选基因,区分了 brojlers 和 layers.
在他们的寿命中观察到层菌株之间的等位基因特异性表达的显著差异.
ASE基因主要参与能量代谢,在峰值产卵期间丰富胆固醇生物合成.

结论:

遗传架构和代谢需求在产卵期间形成的等位基因异质.
对等位基因特异性的基因调控是理解基因型-表型映射和功能多样性的关键.
ASE和FST之间的重叠表明基因在cis-regulatory元素中的固定,受育种和管理的影响.

关键词:

在RNA-seqq.国家统一计划 (SNP) 是一个国家统一计划.亚ليل特异性表达的表达方式 brojler 肉肉是一个固定指数的固定指数产卵是一个产卵.

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