长非编码RNA DSCAS通过与miR-646-3p竞争性结合来调节肺状细胞癌中的思丁敏感性
Hongping Liu1,2, Chunya Lu1, Ping Li1
1Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, No.1, Jianshe East Road, Zhengzhou, Henan 450052, PR China.
Heliyon
|June 26, 2023
概括
研究人员确定lncRNA DSCAS是肺状细胞癌 (LUSC) 抗西斯的关键因素. 上调的DSCAS促进LUSC的进展和抵抗,而它的下调抑制这些效应.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 基于的化疗是晚期肺状细胞癌 (LUSC) 的标准.
- 西斯普拉丁耐药性是LUSC治疗的一个主要挑战,影响患者的预后.
- 识别新的治疗点,如特定的 lncRNA,对于克服耐药性至关重要.
研究的目的:
- 调查长非编码RNA DSCAS (DSCAS) 在LUSC.中西斯普拉丁耐药性的发展中的作用.
- 阐明DSCAS影响LUSC细胞行为和药物敏感性的分子机制.
主要方法:
- 使用lncRNA微阵列和qPCR进行差异表达查.
- 功能性研究涉及使用lentiviral转染来调节DSCAS表达.
- 通过各种测定 (CCK-8,殖民地形成,伤口愈合,Transwell,流细胞计) 来评估细胞增殖,入侵,迁移和对西斯普拉丁的敏感性.
- 使用双化酶记者,RNA-IP和RNA-pull-down测定来研究分子相互作用,其次是西方抹杀和qPCR用于途径验证.
主要成果:
- DSCAS表达在LUSC组织和细胞中升高,与西斯普拉丁不敏感相关.
- DSCAS的升级增强了LUSC细胞的增殖,迁移和入侵,同时增加了对西斯普拉丁的耐药性.
- 低调 DSCAS 抑制了这些恶性行为,并降低了对西斯的抗药性.
- DSCAS与miR-646-3p直接相互作用,调节与亡相关的蛋白Bcl-2和Survivin的表达.
结论:
- DSCAS在调节LUSC细胞的生物行为和对西斯普拉丁敏感性方面发挥着重要作用.
- 该机制涉及DSCAS与miR-646-3p竞争性结合,从而影响Survivin和Bcl-2表达.
- 准DSCAS可能提供一种潜在的治疗策略,以克服LUSC中西斯普拉丁耐药性.
相关概念视频
01:22MicroRNAs
21.4K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.4K
02:39lncRNA - Long Non-coding RNAs
8.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K
02:15Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
01:23RNA Interference
26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K
02:30siRNA - Small Interfering RNAs
16.9K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.9K