Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Decoding the impact of nsSNP variants on BCL6 function through integrated computational analysis.

Current research in structural biology·2026
Same author

Synthesis of Pluronic F127 copolymer/iron oxide-GelMA nanocomposite for doxorubicin drug delivery.

Nanoscale advances·2026
Same author

Corrigendum to "Exosomes as novel tools for renal cell carcinoma therapy, diagnosis, and prognosis" [Heliyon Volume 10, Issue 12, June 30, 2024, Article e32875].

Heliyon·2025
Same author

Enhanced Alginate Microparticles Fabricated by a Microfluidic System for Local Mesalamine Delivery to Inflammatory Bowel Disease.

Biomacromolecules·2025
Same author

Identification of effective inhibitors against epsilon toxin (ETX) of Clostridium perfringens: Virtual screening and molecular dynamics simulation.

Toxicon : official journal of the International Society on Toxinology·2025
Same author

Current Understanding of the Exosomes and Their Associated Biomolecules in the Glioblastoma Biology, Clinical Treatment, and Diagnosis.

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology·2025

相关实验视频

Updated: Jul 25, 2025

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis
08:33

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis

Published on: August 11, 2022

2.4K

使用微流体装置对HER2阳性乳腺癌细胞进行免疫磁隔离.

Delaram Parvin1, Zahra Sadat Hashemi2, Farhad Shokati3

  • 1School of Mechanical Engineering, College of Engineering, University of Tehran, North Amirabad, 1439957131 Tehran, Iran.

ACS omega
|June 26, 2023
PubMed
概括

这项研究开发了一种低成本的微流体芯片,使用磁纳米颗粒来隔离HER2阳性乳腺癌细胞. 该系统实现了96%的隔离效率,为个性化癌症诊断和治疗监测提供了一个有前途的工具.

更多相关视频

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.2K
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

4.8K

相关实验视频

Last Updated: Jul 25, 2025

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis
08:33

Microfluidic Device for the Separation of Non-Metastatic MCF-7 and Non-Tumor MCF-10A Breast Cancer Cells Using AC Dielectrophoresis

Published on: August 11, 2022

2.4K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.2K
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

4.8K

科学领域:

  • 生物医学工程 生物医学工程
  • 纳米技术 纳米技术
  • 癌症研究 癌症研究

背景情况:

  • 循环瘤细胞 (CTC) 是监测转移性癌症的关键生物标志物,可为个性化癌症治疗提供早期诊断和预后评估.
  • 有效,可行和低成本的CTC隔离方法对于推进临床应用至关重要.
  • HER2阳性乳腺癌需要特定的检测和隔离策略.

研究的目的:

  • 开发和验证一个集成的微流体系统,利用磁纳米粒子 (MNP) 来有效隔离HER2阳性乳腺癌细胞.
  • 评估与现有方法相比,在隔离效率,特异性和速度方面开发系统的性能.
  • 为CTC隔离提供具有竞争力和临床适用性的解决方案.

主要方法:

  • 用抗HER2抗体功能化的氧化铁MNP的合成和表征.
  • 使用富里埃变换红外光谱学,能量分散式X射线光谱学和动态光散射/泽塔电位分析来验证化学结合.
  • 使用具有S形微通道的微流体芯片在芯片外和芯片内设置中证明特定的HER2阳性细胞隔离.

主要成果:

  • 在HER2阳性细胞的离芯片隔离效率为59.38%.
  • 使用微流体芯片的芯片内隔离效率达到96%的SK-BR-3细胞在0.5毫升/小时的流量,而没有芯片堵塞.
  • 与传统方法相比,芯片上的细胞分离时间减少了50%.

结论:

  • 集成的微流体系统与抗HER2功能化的MNP显示出高效率和特异性来隔离HER2阳性乳腺癌细胞.
  • 这种方法比现有方法有了显著的改进,为CTC分析提供了更快,无堵塞的解决方案.
  • 开发的系统为个性化癌症诊断和治疗监测的临床应用提供了一个具有竞争力和有前途的工具.