在病毒性菌体疾病复杂形成期间,快速抑制防御机制
Amit M Philosoph1,2, Aviv Dombrovsky1, Neta Luria1
1Department of Plant Pathology and Weed Science, The Volcani Institute, Agricultural Research Organization, Bet Dagan, Israel.
Frontiers in plant science
|June 26, 2023
概括
黄瓜植物感染了死菌病原体 (Pythium spinosum) 和病毒 (黄瓜绿色斑点马赛克病毒) 显示了抑制的防御机制. 这种病毒共感染增加了对真菌病原体的敏感性,导致严重的疾病和植物死亡率.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 文字转录学 (Transcriptomics) 是一个学科.
背景情况:
- 与不同寄生虫生活方式的病原体结合感染可以导致协同效应,加剧植物疾病.
- 了解宿主对并发感染的反应对于疾病管理至关重要.
- 黄瓜植物对真菌和病毒病原体都敏感,影响农业产量.
研究的目的:
- 调查黄瓜植物在单一和联合感染期间与Pythium spinosum (非营养性) 和黄瓜绿斑马马赛克病毒 (CGMMV,生物营养性) 的转录基因变化.
- 为了阐明分子动力学和宿主防御反应在并发的病原体攻击期间.
- 确定潜在的协同作用相互作用及其对疾病严重程度的影响.
主要方法:
- 对遭受单次感染 (P. spinosum或CGMMV) 和并发感染的黄瓜植物进行转录基因分析.
- 在注射后的各个点进行时间过程采样.
- 基因表达分析,以评估宿主防御机制.
主要成果:
- 单独的CGMMV感染对宿主基因表达有轻微的影响,而P. spinosum则导致了剧烈的变化.
- 与CGMMV的同时感染,在病毒接种后24小时内就迅速降低了对P. spinosum的宿主防御基因的调节.
- 同时感染的植物中抑制的防御导致P. spinosum hyphae增加,严重的压力和30%的植物死亡率,只有在13天后才观察到防御恢复.
结论:
- 病毒感染 (CGMMV) 颠覆了Pythium预先感染的植物的宿主防御系统,改变了既定的平衡.
- 在CGMMV感染后,存在一个特定的时间窗口,增加对P. spinosum的敏感性.
- 这些发现强调了不同类型的病原体之间的复杂相互作用及其对宿主免疫力的影响.
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