Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Flow Cytometry01:23

Flow Cytometry

13.2K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Ergothioneine-rich water extracts of <i>Hericium erinaceus</i> HE-17 alleviate Alzheimer's disease in mice by regulating oxidative stress, inflammation, and the gut microenvironment.

Frontiers in nutrition·2026
Same author

Calcium signaling mediated by glutamate receptor-like protein PagGLR3.3 is involved in tension wood induction in poplar.

Journal of integrative plant biology·2026
Same author

PagARF3.1 promotes adventitious root formation by repressing <i>IPT</i>-mediated cytokinin biosynthesis.

Forestry research·2025
Same author

Metabolism-dependent succinylation governs resource allocation for antibiotic resistance.

Science advances·2025
Same author

Three New Physalins from <i>Physalis Alkekengi</i> L. var. <i>franchetii</i> (Mast.) Makino.

Molecules (Basel, Switzerland)·2025
Same author

A Multicentre Clinical Trial Protocol of Shuganjieyu Capsules Combined With Fluoxetine in the Treatment of Depression.

Cureus·2025

相关实验视频

Updated: Jul 25, 2025

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
13:53

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Published on: August 26, 2021

4.6K

一种通过流细胞计量来分析细胞发育中的编程细胞死亡的方法.

Ying-Li Liu1, Ying-Hua Guo2, Xue-Qin Song3

  • 1State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China.

Frontiers in plant science
|June 26, 2023
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的流细胞计法,用于研究木质植物的编程细胞死亡 (PCD). 这种技术有效地区分活细胞,早期细胞和晚期PCD细胞,有助于木材形成研究.

关键词:
传真 (FACS) 是一个事实.这是一个PCDPCD.基因表达的基因表达方式有木质植物的木质植物.克西勒姆的发展发展.

更多相关视频

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
07:32

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish

Published on: April 29, 2021

5.5K
Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response
06:06

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Published on: November 10, 2023

1.4K

相关实验视频

Last Updated: Jul 25, 2025

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
13:53

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Published on: August 26, 2021

4.6K
Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
07:32

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish

Published on: April 29, 2021

5.5K
Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response
06:06

Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response

Published on: November 10, 2023

1.4K

科学领域:

  • 植物生物学 植物生物学
  • 细胞生物学 细胞生物学
  • 木材科学 木材科学 木材科学

背景情况:

  • 编程细胞死亡 (PCD) 对植物发育至关重要,包括木材形成.
  • 由于缺乏有效的方法,研究木质植物的PCD具有挑战性.
  • 流细胞计是哺乳动物亡的常见方法,但在植物中未得到充分利用.

研究的目的:

  • 建立一种有效的方法来研究木质植物的编程细胞死亡 (PCD).
  • 为分析植物细胞死亡而适应流细胞计技术.
  • 促进对木材形成的分子机制的研究.

主要方法:

  • 从树茎中分离出来的西细胞原质体.
  • 染色原生体与光素附件V-FITC和化 (PI).
  • 使用光激活细胞分类 (FACS) 进行细胞分析.

主要成果:

  • 通过使用FACS.成功区分活细胞,早期PCD和晚期PCD细胞.
  • 实现了细胞群的定量分析.
  • 基因表达模式与细胞形态和死亡阶段相关.

结论:

  • 一种新的FACS方法有效地研究木质植物中的PCD.
  • 这种方法为研究木材形成提供了有价值的工具.
  • 该技术增强了我们对植物发育过程的理解.