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人类真菌病原体LAMP试验的诊断效率:系统性审查和元分析

Gurvinder Singh Bumbrah¹, Sarika Jain², Shweta Singh³

¹Department of Forensic Sciences, Amity School of Applied Sciences, Amity University Haryana, Gurugram, 122413 Manesar India.

Current fungal infection reports

|June 26, 2023

概括

循环介导异热放大 (LAMP) 为人类真菌感染提供了快速,准确的诊断方法,性能优于常规测试. 这一元分析证实了LAMP的存在.

科学领域:

医学真菌学医学真菌学
诊断微生物学诊断微生物学
分子诊断学分子诊断

背景情况:

由Candida和Aspergillus引起的人类真菌感染构成了重大的公共卫生挑战.
对于真菌病原体的常规诊断方法,需要很长的周转时间和低灵敏度.
现有的分子诊断是敏感的,但需要专门的基础设施和昂贵.

研究的目的:

进行元分析,评估循环介导同热放大 (LAMP) 用于检测人类真菌病原体的诊断效率.
评估LAMP作为一种快速,准确和广泛采用的替代现有诊断方法.
遵守PRISMA系统审查和元分析指南.

主要方法:

在PubMed,谷歌学者,科学直接,Scopus,BioRxiv和MedRxiv的系统文献搜索.
包括9项符合条件的研究,符合基于LAMP的真菌诊断标准.
使用QUADAS-2进行质量评估,以确定偏差和适用性.

主要成果:

分析主要包括来自中国和日本的研究,使用唾液和血液样本.
内部转录间隔器 (ITS) 基因和基于光的检测是常见的目标和方法.
聚合灵敏度在0.71到1.0之间,聚合特异性在0.13到1.0之间 (95% CI).
准确度和精度分别在70-100%和68-100%之间.
研究表明,偏见和适用性问题风险较低.

结论:

循环介导异热放大 (LAMP) 是快速真菌诊断的可行和有前途的替代方案.
灯光技术可以促进广泛采用,特别是在资源有限的环境中.
可能需要进一步验证,但LAMP显示有可能解决当前真菌诊断方法的局限性.

关键词:

亚斯珀吉洛斯 (Aspergillus) 是一种植物.这是一种Candida Candida.诊断诊断诊断诊断诊断菌的真菌是一种一个灯的灯光.进行元分析分析.

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