在Nostoc sp.中适应缺乏的调节反感转录. 在PCC 7120中
Manuel Brenes-Álvarez1, Agustín Vioque1, Alicia M Muro-Pastor1
1Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, Américo Vespucio 49, 41092 Sevilla, Spain.
PNAS nexus
|June 26, 2023
概括
研究人员绘制了Nostoc sp.的转录组图. PCC 7120,发现了许多反意义RNA,包括由NtcA和HetR调节的新型非编码RNA,可能影响异构体分化.
科学领域:
- 细菌的转录组学
- 蓝藻细菌生物学的生物学
- 基因调节 基因调节
背景情况:
- 高通量转录组分析揭示了细菌中广泛存在的反意义转录.
- 反理性RNA可以由重叠的编码序列产生的,也可以是非编码的.
- 诺斯托克公司Nostoc sp. 在限制下,PCC 7120表现出细胞类型分化 (植物性细胞和异构体),由NtcA和HetR调节.
研究的目的:
- 为了确定参与Nostoc sp.异构体分化中的反感性RNAs. 在PCC 7120.
- 构建Nostoc sp.的一个全面的转录地图. 在限制下PCC 7120.
主要方法:
- 对Nostoc sp. 的RNA测序 (RNA-seq) 分析 在限下PCC 7120 (除后9个和24小时).
- 整合全基因组的转录起点和预测的转录终结点.
- 对异质囊中的反感性RNA (as_gltA) 功能和表达的分析.
主要成果:
- 创建了一个超过4,000个转录的转录地图,其中65%的转录显示出反意义方向.
- 鉴定了从NtcA或HetR依赖促进体转录的新型调节的非编码抗意义RNA.
- 发现针对异体细胞的反感性RNA as_gltA 在过度表达时降低了酸盐合成酶活性.
结论:
- 在Nostoc sp.中,反意义转录丰富多样. 在PCC 7120.
- 存在由关键异构细胞分化因子 (NtcA,HetR) 调节的非编码反感RNA.
- as_gltA代表了在异质囊发育期间代谢重塑中的潜在调节者.
相关概念视频
Stringent Response in E. coli
32
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
32
Transcription Attenuation in Prokaryotes
15.6K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.6K
Translational Regulation
45
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
45
Inorganic Nitrogen Assimilation
49
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
49
Prokaryotic Transcriptional Activators and Repressors
21.2K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.2K
Types of RNA
64.1K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
64.1K


