间歇性禁食可持续改善正常体重雄性小鼠的葡萄糖耐受性,通过质子过乙化
Sho Endo1, Asuka Uto1, Kazutoshi Miyashita1,2
1Department of Internal Medicine, Keio University School of Medicine, Tokyo, 160-8582, Japan.
间歇性禁食 (IF) 通过增加肌肉中的脂质利用来促进持续的代谢益处. 这种效应与关键基因的组素乙化有关,即使在禁食停止后.
科学领域:
- 代谢生理学 代谢生理学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 已知间歇性禁食 (IF) 会影响新陈代谢.
- 断药后IF的长期影响尚未完全理解.
- 了解持续的IF效应背后的分子机制对于代谢健康至关重要.
研究的目的:
- 研究一种特定的间歇性禁食对骨肌肉代谢的持续影响.
- 探索基因素乙化在介导IF引起的长期代谢变化的作用.
- 为了比较72小时禁食周期与隔日禁食的代谢影响.
主要方法:
- 小鼠接受了4个周期的72小时禁食,随后进行96小时的食 (72hIF).
- 分析了与脂质新陈代谢和骨肌肉中的基因乙化相关的基因表达.
- 代谢参数在IF停药期间和停药后4周内进行评估.
主要成果:
- 72hIF疗法诱导了显著的代谢重塑,增强了骨肌肉中的脂质利用和线粒体活性.
- 在IF后4周观察到脂肪氧化基因持续升高调节和氧气消耗增加.
- 对*Ucp3*和*Cpt1b*基因促进体的素过乙化与持续的代谢效应相关.
- 氨酸水平的增加,可能来自肌肉降解,与基因素乙化有关.
结论:
- 长期间歇性禁食 (72hIF) 会导致骨肌肉的持续代谢改善.
- 在IF期间和之后,肌肉和脂肪组织中脂质氧化基因的希斯乙化有助于持久的代谢效益.
- 这种表观遗传修饰为间歇性禁食的持久影响提供了新的视角.
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