微生物组分析揭示了通过以多糖为基础的益生菌养Oreochromis niloticus (林奈斯,1758) 的肠道微生物群调节的潜力
Asit Kumar Bera1, Hemanta Chowdhury1, Sandeep Ghatak2
1Central Inland Fisheries Research Institute (ICAR), Bārākpur, India.
Frontiers in physiology
|June 26, 2023
概括
作为一种益生菌, Vera 提取物显著改变了尼罗河鱼 (O. niloticus) 的肠道微生物群. 更高的剂量促进了有益的细菌,并增强了改善鱼类健康的关键代谢途径.
科学领域:
- 水生微生物学 水生微生物学
- 鱼的营养 鱼的营养
- 肠道微生物组分析
背景情况:
- 了解鱼的肠道微生物群对于优化营养和宿主微生物相互作用至关重要.
- 基于多糖的益生菌,如叶提取物,显示出在水产养殖中调节肠道健康的潜力.
研究的目的:
- 描述尼罗河鱼 (O. niloticus) 的肠道微生物群落.
- 为了评估不同剂量的叶提取物对肠道微生物组合和功能的影响.
- 评估作为改善鱼类生长性能的益生菌的潜力.
主要方法:
- 一个为期30天的养试验,用尼罗河鱼补充了0.5%和0.75%的阿洛韦拉提取物.
- 在Illumina HiSeq 2500平台上使用16S rRNA基因测序 (V3-V4区域) 的基因组分析.
- 生物信息分析用于操作分类学单位 (OTU) 识别和预测功能分析 (PICRUSt).
主要成果:
- 确定了主导类型:浮游生物,虫类,蛋白质细菌,虫微生物,动态细菌和菌.
- 较高的阿洛韦拉剂量增加了浮游生物和虫,同时减少了蛋白质细菌和虫微生物.
- 观察到大量的细菌,Bacillaceae和Bacillus细菌的数量显著增加.
- 预测的功能分析显示,随着益生菌剂量的增加,高调 glycolysis 和碳水化合物代谢途径.
结论:
- 用叶提取物补充饮食可以有效调节尼罗河鱼的肠道微生物群.
- 该研究强调了植物性多糖类作为益生菌的潜力,可以提高商业鱼类的肠道健康和潜在的生长性能.
- 通过有针对性的营养干预来优化肠道微生物群落是水产养殖的一个有希望的战略.
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