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转移性和非转移性结直肠癌之间的组织相关细菌的差异

Peng Zhou^1,2, Ze Dai^1,2, Yaoyao Xie³

¹Department of Gastroenterology, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, China.

Frontiers in microbiology

|June 26, 2023

概括

组织相关的微生物群在转移性和非转移性结直肠癌 (CRC) 之间存在差异. 在转移性CRC组织中增加的细菌和减少的链球菌可能有助于诊断和治疗CRC转移.

科学领域:

微生物学微生物学
在瘤学瘤学.
胃肠病学胃肠病学

背景情况:

确定了肠道微生物群在结直肠癌 (CRC) 发病和进展中的作用.
组织相关微生物群对CRC转移的影响仍然不太清楚.

研究的目的:

为了研究转移性和非转移性CRC组织之间的细菌差异.
为了识别与CRC转移相关的细菌物种.

主要方法:

从转移性 (n=48) 和非转移性 (n=44) CRC 患者的肠道组织相关微生物群的16S rDNA 扩增高通量测序.
使用标准微生物学方法和qPCR确认分析微生物多样性和差异性物种.
使用接收器操作特征 (ROC) 曲线对CRC转移的细菌预测能力的评估.

主要成果:

转移性和非转移性CRC群体之间的α或β多样性没有显著差异.
在转移组中,细菌和脱硫细菌的丰度明显高,蛋白质细菌的丰度较低.
与非转移性组织相比,转移性CRC组织中的Bacteroides属增加和Streptococcus和Escherichia-Shigella减少.
ROC分析显示,当与CEA结合时,选择细菌的AUC值从0.598到0.69不等,增加到0.678-0.705.
瘤邻近组织中的不同细菌反映了瘤组织中的发现.

结论:

转移性和非转移性CRC组织以及相邻组织之间的细菌组成不同.
升高的细菌菌和降低的链球菌是区分转移性CRC的关键指标.
结果提供了对CRC转移机制和潜在的临床诊断/治疗点的见解.

关键词:

细菌菌菌类的存在链球菌是一种链球菌.大肠直肠癌 (CRC) 是一种癌症.转移转移转移转移与组织相关的细菌.

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