Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.5K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.5K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Characterization of Fluorophore-Quencher Pairs for Distance-Dependent Molecular Sensing in Photoacoustic Imaging.

Chemical & biomedical imaging·2026
Same author

Exploring the Photoswitching Dynamics of Azobenzene Salts and their Role in Inducing Wormlike Micelle Formation in Cationic Surfactants.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Integrative proteomics and lipidomics reveals dual roles for lipid droplets in the host cell antiviral response.

Nature communications·2026
Same author

Repulsive Gas-Electrode van der Waals Forces Enable Charge Transfer Reactions under Chemically Modified Bubbles.

Journal of the American Chemical Society·2026
Same author

Harnessing Methyltransferase-Guided Targeting for Sequence-Specific Proximity Labeling of DNA.

Angewandte Chemie (International ed. in English)·2026
Same author

KRAS Can Bind to FTase Despite Disruption of the CAAX Binding Site.

Biochemistry·2026

相关实验视频

Updated: Jul 25, 2025

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

9.8K

活细胞SOFI与SMLM和AFM成像相关.

Riley B Hargreaves1, Sam Duwé2, Ashley M Rozario3

  • 1School of Chemistry, Monash University, Melbourne, Victoria 3800, Australia.

ACS bio & med chem Au
|June 26, 2023
PubMed
概括

活细胞超分辨率光学波动成像 (SOFI) 与固定细胞技术相结合,以验证细胞结构的成像. 这种相关显微镜方法在样本准备过程中证实了最小的工件,提高了活细胞成像可靠性.

更多相关视频

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy
07:40

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

1.4K
Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

1.0K

相关实验视频

Last Updated: Jul 25, 2025

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
09:09

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

9.8K
A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy
07:40

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

1.4K
Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

1.0K

科学领域:

  • 细胞和分子成像技术
  • 生物物理学的生物物理.
  • 超高分辨率的显微镜

背景情况:

  • 标准的光学显微镜被衍射限制,阻碍了亚细胞组件的可视化.
  • 超分辨率技术提供更高的分辨率,但通常需要固定样本,这引发了关于图像保真性的问题.
  • 相对显微镜结合了多种技术来克服个人限制.

研究的目的:

  • 将活细胞超分辨率光学波动成像 (SOFI) 集成到与单分子定位显微镜 (SMLM) 和原子力显微镜 (AFM) 的相关工作流中.
  • 通过比较活细胞SOFI与固定细胞SMLM和AFM来评估样本制备对细胞超结构的影响.
  • 为了评估双重目标活细胞SOFI的光蛋白组合.

主要方法:

  • 在COS-7细胞上使用低激光功率和光蛋白进行了活细胞SOFI.
  • 使用SOFI,SMLM和AFM进行了相关成像,以分析微管和行为体.
  • 测试了各种光蛋白对的双目标SOFI能力.

主要成果:

  • 微管的活细胞SOFI在固定后20分钟内显示微管网络的最小变化.
  • SOFI,SMLM和AFM提供了微管子尺寸和突起的补充测量.
  • 特定的光蛋白组合 (rsGreen1-rsKAME,rsGreen1-Dronpa, ffDronpaF-rsKAME) 已被验证为两个目标SOFI.

结论:

  • 相对的SOFI-SMLM-AFM证明了活细胞超高分辨率成像的可行性,使用最小的样本准备工件.
  • 这种综合方法通过与活细胞成像相比,验证固定细胞超分辨率数据的准确性.
  • 这项研究强调了相关显微镜在活细胞和固定细胞中可靠的超结构分析的力量.