在 (Chrysopogon zizanioides) 根转录组中识别和验证参考基因
Abhishek Singh Chauhan1,2, Madhu Tiwari2, Yuvraj Indoliya2
1Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002 India.
概括
这项研究确定了 (Vetiveria zizanioides) 中最好的参考基因,用于准确的基因表达分析. 在各种非生物压力下验证了五个最佳基因,包括SAMS和Core histone 1.
科学领域:
- 分子生物学分子生物学
- 植物科学 植物科学
- 基因组学就是基因组学.
背景情况:
- 草 (Vetiveria zizanioides) 是一种有价值的C-4草,用于植物修复和土壤/水资源保护.
- 它的深层根系使其对环境应用至关重要.
- 准确的基因表达分析对于了解的特征至关重要.
研究的目的:
- 为了识别和验证稳定的参考基因在根.
- 为了支持在非生物压力条件下准确的基因表达研究.
- 为转录和分子数据分析提供质量控制措施.
主要方法:
- 分析了来自两个形形态型 (北印度和南印度) 的转录组数据.
- 在新的组装中确定了136,824个基因.
- 多种统计工具 (RefFinder,NormFinder,BestKeeper,geNorm,Delta-Ct) 和qRT-PCR被用于评估11个候选基因.
主要成果:
- 五个参考基因 (GAPs,OsCam2,MUB,核心基因素1,SAMS) 以及阿克丁被确定为最好的基因.
- 通过使用qbase+软件,SAMS和核心基因素1被确定为两个最优的参考基因.
- 基因表达稳定性在热,寒,盐和干旱压力中得到验证.
结论:
- 已识别的参考基因对于精确的正常化在虫基因表达研究中至关重要.
- 这些发现将有助于分析与非生物压力和发育相关的表型.
- 这项研究为涉及的分子实验的质量控制提供了必要的工具.
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