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在非小细胞肺癌中探索HER2 (ERBB2) 免疫组织化学:与ERBB2突变状态的相关性:实验研究实验研究

Anthony B Cardillo¹, Sierra Kovar¹, Nitin Roper²

¹Department of Pathology and Laboratory Medicine, University of Rochester, Rochester, NY.

Annals of medicine and surgery (2012)

|June 26, 2023

概括

在肺癌中,ERBB2 (HER2) 突变是罕见的. 虽然可以检测ERBB2基因放大,但目前的商业抗体难以准确识别非小细胞肺癌中的ERBB2突变.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

ERBB2 (HER2) 基因编码了一种表皮生长因子受体家族中的蛋白质.
在非小细胞肺癌 (NSCLC) 中,ERBB2突变不常见.
临床试验正在研究ERBB2变异的NSCLC患者的向疗法和无进展生存率.

研究的目的:

评估商用ERBB2 (HER2) 免疫组织化学抗体的诊断准确性.
评估下一代测序 (NGS) 和免疫组织化学 (IHC) 之间的一致性,以检测肺腺癌中的ERBB2变异.

主要方法:

恢复的肺腺癌病例与NGS证明的ERBB2点突变 (n=8) 或放大 (n=11).
评估了NGS结果与商用ERBB2 (HER2) IHC抗体之间的一致性.

主要成果:

没有商业上可用的ERBB2抗体克隆可以在NSCLC标本中始终准确地检测到ERBB2突变.
使用商业上可用的抗体,用合理的诊断准确度检测到ERBB2放大.

结论:

目前的ERBB2 (HER2) IHC测定显示在精确检测NSCLC中的ERBB2突变方面存在局限性.
ERBB2 (HER2) IHC可能适用于检测NSCLC的放大,但不能检测NSCLC的突变.

关键词:

在ERBB2中,它是ERBB2.在HER2中,HER2是HER2.免疫组织化学免疫组织化学非小细胞肺癌的肺癌.

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