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骨髓中介质干细胞扩展到状小支架内:关注抗炎反应

Bianca Barzaghini¹, Stephana Carelli^2,3, Letizia Messa^3,4

¹Department of Chemistry, Materials, and Chemical Engineering "Giulio Natta," Politecnico Di Milano, Milan, Italy.

Regenerative engineering and translational medicine

|June 26, 2023

概括

在3D尼状支架中培养的介质干细胞 (MSC) 保持静止状态并增强免疫反应基因. 这支持它们在治疗儿科肺部疾病,包括COVID后疾病方面的潜力.

科学领域:

再生医学是一种再生医学.
生物医学工程生物医学工程
细胞生物学细胞生物学

背景情况:

介质细胞干细胞 (MSCs) 由于其低免疫性和抗炎性质,对儿科疾病具有治疗潜力.
多重复合体通常居住在一个专门的微环境中,这会影响它们的功能.
标准的体外扩张可以导致干细胞分化和再生潜力的丧失.

研究的目的:

为了研究3D微型支架 (Nichoid) 的效果,该支架模拟了儿科骨髓MSCs (BM-MSCs) 上的本地利基.
为了评估是否机械刺激内Nichoid可以增强MSC抗炎潜力没有生物化学因素.
评估Nichoid作为临床翻译的创新培养系统的适用性.

主要方法:

在3D Nichoid 支架内扩展儿科 BM-MSC.
综合细胞表征包括活力测试,免疫光,RNA测序和基因表达分析.
在Nichoid中的BM-MSCs与传统的2D培养 (平面控制) 的比较.

主要成果:

在Nichoid中培养的BM-MSC保持了静止干细胞状态,模仿了体内环境.
与平面对照相比,在Nichoid内的BM-MSC中观察到与免疫反应相关的基因的显著上调.
三维支架仅通过机械刺激增强了抗炎特征.

结论:

尼科伊德支架提供了一个适合的微环境来扩展MSC,保持它们的茎状性并增强它们的治疗性质.
观察到的基因表达变化表明潜在的治疗效果,特别是对于抗炎功能.
这些发现支持基于MSC的疗法用于儿科肺部疾病的临床转化,包括与COVID-19相关的疾病.

关键词:

工程干细胞是一个利基领域.介质细胞干细胞介质细胞干细胞尼科伊德脚手架是一个尼科伊德脚手架再生医学是一种再生医学.

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