三维培养条件骨髓MSC秘密加速伤口愈合在一个烧伤伤害小鼠模型
Prakash Gangadaran1, Eun Jung Oh2, Ramya Lakshmi Rajendran3
1BK21 FOUR KNU Convergence Educational Program of Biomedical Sciences for Creative Future Talents, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu, 41944, South Korea; Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu, 41944, South Korea.
Biochemical and biophysical research communications
|June 26, 2023
概括
在烧伤模型中,三维培养介质干细胞 (MSCs) 增强了它们的分泌体,促进了血管生成并加速了伤口愈合. 这种3D-MSC方法为再生医学提供了一种基于细胞的新疗法.
科学领域:
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
- 伤口愈合 治愈 伤口愈合
背景情况:
- 基于介质细胞干细胞 (MSC) 的疗法在伤口愈合方面表现有前途.
- 了解MSC培养方法如何影响其治疗潜力至关重要.
研究的目的:
- 为了比较2D单层 (2D-MSCs) 与3D球形 (3D-MSCs) 中培养的MSCs的分泌体.
- 评估3D-MSC分泌体对内皮细胞 (EC) 的影响.
- 评估2D-MSC和3D-MSC在烧伤小鼠模型中的有效性.
主要方法:
- MSCs被培养为2D单层和3D球形.
- 西方涂抹评估了细胞特征.
- 分泌体 (有条件的介质) 分析了血管生成因子.
- 通过增殖,迁移和管形成测试来测试EC激活.
- 在活体中,伤口愈合在烧伤小鼠模型中进行了评估.
主要成果:
- 3D培养改变了与细胞存活,细胞骨,粘附和增殖相关的标记.
- 与2D-MSC秘密体相比,3D-MSC秘密体显示了较高的IL-6,VEGFA,IL-8和CXCL1水平.
- 3D-MSC分泌体显著增强了EC的增殖,迁移和管道形成.
- 在烧伤模型中,3D-MSC治疗改善了伤口愈合.
结论:
- 3D培养增强了MSC分泌体中的益血管性因素.
- 3D-MSCs代表了一种有前途的基于细胞的治疗策略,用于伤口愈合.
- 这项研究强调了3D细胞培养在优化MSC基础再生疗法的潜力.
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