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外源谷氨可以通过激活抗氧化系统和调节DNA甲基化来缓解kenaf中的毒性

Meiqiong Tang¹, Ru Li², Peng Chen³

¹Guangxi Key Laboratory of Agro-environment and Agric-products Safety, Key Laboratory of Crop Genetics Breeding and Germplasm Innovation, College of Agriculture, Guangxi University, Nanning, 530004, China; Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, National Center for TCM Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China.

|June 26, 2023

概括

外源性谷氨 (GSH) 通过增加DNA甲基化,增强抗氧化防御和改善基因表达来增强kenaf中的 (Cr) 耐受性. 这项研究揭示了GSH.

科学领域:

植物生物学植物生物学
环境毒理学环境毒理学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

谷氨 (GSH) 对于植物重金属 (HM) 应激反应至关重要.
在HM排毒中GSH的表观遗传调节仍然不太了解.
(Cr) 压力严重影响植物生长和生理学.

研究的目的:

阐明GSH在kenaf对Cr压力的反应中的表观遗传机制.
为了研究DNA甲基化在GSH介导的Cr排毒中的作用.
确定参与CR耐受性和其调节的关键基因.

主要方法:

在Cr压力下,Kenaf苗木被用和没有GSH处理.
用生理分析,qRT-PCR和MethylRAD-seq进行了全面分析.
对像HcTrx这样的差异甲基化基因 (DMG) 进行了功能分析.

主要成果:

治疗GSH缓解了Cr诱导的生长抑制和氧化损伤.
GSH调节了DNA甲基转移酶和脱甲基酶基因表达,增加了DNA甲基化水平.
不同甲基化基因在DNA修复和氧化还原酶活性中得到丰富;HcTrx过度表达增强了Cr耐受性.

结论:

外源的GSH通过调节DNA甲基化模式来增强kenaf中的Cr排毒.
由GSH介导的表观遗传变化激活了抗氧化剂防御系统,从而提高了CR耐受性.
具有特征的Cr耐受性基因HcTrx在kenaf繁殖中提供了基因改进的潜力.

关键词:

应激应变是因为的应激.通过DNA甲基化.排毒排毒排毒排毒这是一种重金属,重金属.在Kenaf的Kenaf.病毒诱导的基因沉默是病毒引起的.

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