油性绿藻用Mortierella alpina真菌进行花
Ty Shitanaka1, Lauren Higa1, Abigail E Bryson2
1Department of Molecular Biosciences & Bioengineering, University of Hawai'i at Mānoa, Honolulu, HI 96822, United States.
Bioresource technology
|June 26, 2023
概括
使用油性真菌Mortierella alpina进行生物花,有效收获微藻. 这种方法增强了从Chlamydomonas reinhardtii突变体中恢复有价值的生物产品,如酸 (ARA).
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 生物工艺工程 生物工艺工程
背景情况:
- 微藻类是生物燃料,食品和营养药品的宝贵来源.
- 由于细胞大小小,生物质密度低,收获微藻很困难.
- 克拉米多马纳斯强硬菌 (sta6/sta7) 的无粉突变体积累了高水平的三糖化物 (TAG).
研究的目的:
- 通过使用油性真菌Mortierella alpina来研究微藻的生物花.
- 了解生物花过程中藻类和真菌相互作用的机制.
- 评估花化生物质的脂肪酸概况.
主要方法:
- 在缺乏的条件下培养无粉微藻突变体 (sta6/sta7).
- 微藻与Mortierella alpina菌株的共同培养.
- 扫描电子显微镜 (SEM) 分析细胞壁附着和细胞外聚合物质 (EPS).
- 气色谱-质谱 (GC-MS) 用于脂肪酸分析.
主要成果:
- 在24小时内,在藻类和真菌生物质比率约为1:1的情况下,达到80-85%的最佳生物花粉效率.
- SEM揭示了细胞壁附着和EPS调解了花过程.
- 叶片化生物质含有相当多的阿拉基酸 (ARA),占总脂肪酸的3-10%.
结论:
- 阿尔卑斯海藻 (Mortierella alpina) 是微藻生物花的一个有希望的候选物.
- 这项研究为改善生物质采集提供了对藻类和真菌相互作用的机制性见解.
- 这种方法促进了微藻生物产品的回收,包括宝贵的脂肪酸,如ARA.
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