增加Acinetobacter baumannii的光消灭效率,使用多色化剂
Irena Maliszewska1, Waldemar Goldeman1
1Department of Organic and Medicinal Chemistry, Faculty of Chemistry, Wrocaw University of Science and Technology, Wybrzeże Wyspiańskiego 27 Wrocław 50-370, Poland.
聚酸如EDTMP,ATMP和龙酸显著增强甲蓝介导的Acinetobacter baumannii的光动力失活. 这些药物可以促进细菌的光去除,特别是与葡萄糖相结合时.
科学领域:
- 微生物学 微生物学
- 摄影化学的使用.
- 生物医学工程 生物医学工程
背景情况:
- 宝曼尼菌 (Acinetobacter baumannii) 是一个重要的机会性病原体.
- 光动力学无活化 (PDI) 是一个有前途的抗菌策略.
- 提高PDI对抗耐药细菌的疗效至关重要.
研究的目的:
- 评估聚酸与甲基蓝 (MB) 介导的PDI对Acinetobacter baumannii的协同作用.
- 为了研究这些剂对细菌的浮游生物和生物膜形式的影响.
- 探索葡萄糖在PDI过程中的作用.
主要方法:
- 用甲蓝和激光照射 (638 nm) 处理了Acinetobacter baumannii的浮游生物和生物膜培养物.
- 细菌被预先化以乙二胺-N,N,N,N'-四基酸 (EDTMP),酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸酸
- 照射时间各不相同 (10,20,30分钟),相当于光剂量为63,126和189 Jcm−2.2.
- 此外,还评估了葡萄糖共养的效果.
主要成果:
- 单独使用MB就能将可活细胞减少3.10 log10单位.
- 采用佐列龙酸,ATMP或EDTMP进行预处理,增强了杀死,使可活细菌减少了大约4.0 log10单位.
- 对于生物膜,EDTMP,ATMP和基酸分别实现了0.65,1.25,0.80log10单位的减少.
- 添加葡萄糖显著促进了光消灭,特别是在生物膜中,ATMP显示出最高的疗效 (3.2 log10减少).
- 多酸增加了光敏剂的结合和细胞脱离.
结论:
- 聚酸,包括EDTMP,ATMP和龙酸,显著增强甲蓝基PDI对抗Acinetobacter baumannii的作用.
- 这些药物有效地减少了浮游生物和生物膜细菌负载.
- 将葡萄糖纳入光敏化系统进一步增强了这种PDI方法的抗菌疗效.
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