对allherpesvirus进行比较基因组分析:探索可用的属/物种划分建议和方法
Wenjie Zhang1, Ran Wang1, Xiaoxia Zou2
1Laboratory Animal Center, Southwest Medical University, Luzhou Sichuan, PR China.
Virus research
|June 26, 2023
概括
这项研究探讨了使用病毒蛋白树和基因组分析进行aloherpesvirus分类. 它为鱼类和两性疹病毒建立了明确的属和物种界限.
科学领域:
- 病毒学 病毒学
- 生物信息学是一种生物信息学.
- 水产养殖健康 水产养殖健康
背景情况:
- 阿洛赫佩斯病毒家族包括影响鱼类和两动物的疹病毒.
- 艾洛赫病毒在水产养殖中造成重大经济损失,推动了对病原和预防的研究.
- 尽管基因组数据越来越多,但目前对艾洛赫佩斯病毒的属/物种分类方法仍不发达.
研究的目的:
- 为了研究艾洛赫珀斯病毒之间的遗传关系.
- 评估和比较各种方法来分类艾滋病病毒.
- 建立强有力的标准,以界定在Alloherpesviridae中的物种和属界限.
主要方法:
- 使用病毒蛋白树 (ViPTree) 对40个完整的alloherpesvirus基因组进行了家族遗传学分析.
- 平均核酸标识 (ANI) 和平均氨基酸标识 (AAI) 分析.
- 核心组基因组分析以确定共享的基因和正统组.
- 对特定的一般性进行点图分析.
主要成果:
- 在ViPTree的分析中,发现了三种不同的单细胞群:Cyprinivirus,Ictalurivirus和Batrachovirus.
- ANI/AAI分析确认了物种界限,具有90%的识别值.
- 核心组分析确定了809个正统组和11个核心基因;15%的身份表明了基因界限.
- 八个核心基因显示出对遗传学分析的潜力.
- 点图分析对伊克塔卢里病毒是有效的,但对赛普里尼病毒或巴特拉科病毒是不有效的.
结论:
- 该研究提供了一个全面的框架,用于allherpesvirus分类.
- 多种分析方法为在不同条件下对这些病毒进行分类提供了多功能选择.
- 建立的标准有助于了解鱼类和两性疹病毒的多样性和分类学.
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