在Aspergillus flavus中发现长非编码RNA对水活动,CO2度和温度变化的反应
Nafiseh Davati1, Abozar Ghorbani2
1Department of Food Science and Technology, College of Food Industry, Bu-Ali Sina University, Hamedan, 65167-38695, Iran. n.davati@basu.ac.ir.
Scientific reports
|June 26, 2023
概括
研究人员在Aspergillus flavus中鉴定出472个长非编码RNA (lncRNA),揭示了它们在调节水活性,CO2和温度等环境压力条件下的细胞功能的关键作用.
科学领域:
- 菌类学 菌类学是指菌类学.
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 长非编码RNAs (lncRNAs) 是动物和植物中确定的调节者,但它们在真菌中的功能不太了解.
- 阿斯伯吉卢斯黄是一种重要的真菌病原体,了解其在压力下的基因调节对于控制策略至关重要.
研究的目的:
- 为了识别和表征阿斯伯吉路斯黄菌中的lncRNAs.
- 在各种环境压力条件下 (水活动,二氧化碳度,温度) 调查lncRNAs的表达模式.
- 预测这些lncRNAs在真菌细胞功能和应激反应中的调节作用.
主要方法:
- 在Aspergillus flavus中对lncRNAs的全基因组识别.
- 在不同的水活性,二氧化碳和温度下对lncRNAs的差异表达分析.
- 预测 lncRNA 功能和亚细胞定位.
主要成果:
- 总共有472个lncRNA被确定,其中470个是新的.
- 在压力条件下观察到 lncRNAs 的显著差异表达.
- 下调的lncRNAs参与调节亚托克辛生物合成,呼吸,生存和新陈代谢.
- 特定的lncRNAs可能会在压力下间接调节proline代谢.
- 在压力下, lncRNAs 主要定位在核中,在高CO2下,在细胞质中发现一些上调的IncRNAs.
结论:
- lncRNAs在Aspergillus flavus适应环境压力的过程中起到关键的调节作用.
- 这些lncRNAs参与了包括新陈代谢和生物合成在内的关键细胞过程.
- 了解lncRNA局部化,可以了解在特定压力条件下它们的调节机制.
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